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  TLR3-Mediated CD8(+) Dendritic Cell Activation Is Coupled with Establishment of a Cell-Intrinsic Antiviral State

Szeles, L., Meissner, F., Dunand-Sauthier, I., Thelemann, C., Hersch, M., Singovski, S., et al. (2015). TLR3-Mediated CD8(+) Dendritic Cell Activation Is Coupled with Establishment of a Cell-Intrinsic Antiviral State. JOURNAL OF IMMUNOLOGY, 195(3), 1025-1033. doi:10.4049/jimmunol.1402033.

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 Creators:
Szeles, Lajos1, Author
Meissner, Felix2, 3, Author              
Dunand-Sauthier, Isabelle1, Author
Thelemann, Christoph1, Author
Hersch, Micha1, Author
Singovski, Simon1, Author
Haller, Sergio1, Author
Gobet, Florian1, Author
Marraco, Silvia A. Fuertes1, Author
Mann, Matthias3, Author              
Garcin, Dominique1, Author
Acha-Orbea, Hans1, Author
Reith, Walter1, Author
Affiliations:
1external, ou_persistent22              
2Meissner, Felix / Experimental Systems Immunology, Max Planck Institute of Biochemistry, Max Planck Society, ou_2149678              
3Mann, Matthias / Proteomics and Signal Transduction, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565159              

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Free keywords: TOLL-LIKE RECEPTOR-3; DOUBLE-STRANDED-RNA; SUBSETS IN-VIVO; TRANSCRIPTIONAL NETWORK; ADAPTIVE IMMUNITY; INFECTED CELLS; FLT3 LIGAND; VIRUS; RESPONSES; INNATE
 Abstract: Because of their unique capacity to cross-present Ags to CD8(+) T cells, mouse lymphoid tissue-resident CD8(+) dendritic cells (DCs) and their migratory counterparts are critical for priming antiviral T cell responses. High expression of the dsRNA sensor TLR3 is a distinctive feature of these cross-presenting DC subsets. TLR3 engagement in CD8(+) DCs promotes cross-presentation and the acquisition of effector functions required for driving antiviral T cell responses. In this study, we performed a comprehensive analysis of the TLR3-induced antiviral program and cell-autonomous immunity in CD8(+) DC lines and primary CD8(+) DCs. We found that TLR3-ligand polyinosinic-polycytidylic acid and human rhinovirus infection induced a potent antiviral protection against Sendai and vesicular stomatitis virus in a TLR3 and type I IFN receptor-dependent manner. Polyinosinic-polycytidylic acid-induced antiviral genes were identified by mass spectrometry-based proteomics and transcriptomics in the CD8(+) DC line. Nanostring nCounter experiments confirmed that these antiviral genes were induced by TLR3 engagement in primary CD8(+) DCs, and indicated that many are secondary TLR3-response genes requiring autocrine IFN-beta stimulation. TLR3-activation thus establishes a type I IFN-dependent antiviral program in a DC subtype playing crucial roles in priming adaptive antiviral immune responses. This mechanism is likely to shield the priming of antiviral responses against inhibition or abrogation by the viral infection. It could be particularly relevant for viruses detected mainly by TLR3, which may not trigger type I IFN production by DCs that lack TLR3, such as plasmacytoid DCs or CD8(-) DCs.

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Language(s): eng - English
 Dates: 2015
 Publication Status: Published in print
 Pages: 9
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: ISI: 000358070400031
DOI: 10.4049/jimmunol.1402033
 Degree: -

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Title: JOURNAL OF IMMUNOLOGY
Source Genre: Journal
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Publ. Info: 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA : AMER ASSOC IMMUNOLOGISTS
Pages: - Volume / Issue: 195 (3) Sequence Number: - Start / End Page: 1025 - 1033 Identifier: ISSN: 0022-1767