English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Sister kinetochore splitting and precocious disintegration of bivalents could explain the maternal age effect.

Zielinska, A. P., Holubcova, Z., Blayney, M., Elder, K., & Schuh, M. (2015). Sister kinetochore splitting and precocious disintegration of bivalents could explain the maternal age effect. eLife, 4: e11389. doi:10.7554/eLife.11389.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002A-1B41-1 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002A-2072-0
Genre: Journal Article

Files

show Files
hide Files
:
2262012.pdf (Publisher version), 4MB
Name:
2262012.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-

Creators

show
hide
 Creators:
Zielinska, A. P., Author
Holubcova, Z., Author
Blayney, M., Author
Elder, K., Author
Schuh, M.1, Author              
Affiliations:
1Department of Meiosis, MPI for Biophysical Chemistry, Max Planck Society, ou_2205654              

Content

show
hide
Free keywords: aneuploidy; cell biology; chromosomes; genes; human; human oocytes; kinetochores; maternal age effect; meiosis; mouse
 Abstract: Aneuploidy in human eggs is the leading cause of pregnancy loss and Down's syndrome. Aneuploid eggs result from chromosome segregation errors when an egg develops from a progenitor cell, called an oocyte. The mechanisms that lead to an increase in aneuploidy with advanced maternal age are largely unclear. Here, we show that many sister kinetochores in human oocytes are separated and do not behave as a single functional unit during the first meiotic division. Having separated sister kinetochores allowed bivalents to rotate by 90 degrees on the spindle and increased the risk of merotelic kinetochore-microtubule attachments. Advanced maternal age led to an increase in sister kinetochore separation, rotated bivalents and merotelic attachments. Chromosome arm cohesion was weakened, and the fraction of bivalents that precociously dissociated into univalents was increased. Together, our data reveal multiple age-related changes in chromosome architecture that could explain why oocyte aneuploidy increases with advanced maternal age.

Details

show
hide
Language(s): eng - English
 Dates: 2015-12-15
 Publication Status: Published online
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.7554/eLife.11389
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: eLife
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: 19 Volume / Issue: 4 Sequence Number: e11389 Start / End Page: - Identifier: -