English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Live imaging RNAi screen reveals genes essential for meiosis in mammalian oocytes.

Pfender, S., Kuznetsov, V., Pasternak, M., Tischer, T., Santhanam, B., & Schuh, M. (2015). Live imaging RNAi screen reveals genes essential for meiosis in mammalian oocytes. Nature, 524(7564), 239-242. doi:10.1038/nature14568.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002A-1B4A-F Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002A-1B53-A
Genre: Journal Article

Files

show Files
hide Files
:
2262019.pdf (Publisher version), 17MB
 
File Permalink:
-
Name:
2262019.pdf
Description:
-
Visibility:
Restricted (Max Planck Institute for Biophysical Chemistry (Karl Friedrich Bonhoeffer Institute), MBPC; )
MIME-Type / Checksum:
application/pdf
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
2262019_Suppl_1.html (Supplementary material), 85KB
Name:
2262019_Suppl_1.html
Description:
-
Visibility:
Public
MIME-Type / Checksum:
text/html / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
2262019_Suppl_2.html (Supplementary material), 85KB
Name:
2262019_Suppl_2.html
Description:
-
Visibility:
Public
MIME-Type / Checksum:
text/html / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
2262019_Suppl_3.xls (Supplementary material), 17MB
Name:
2262019_Suppl_3.xls
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/vnd.ms-excel / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
2262019-Suppl_4.xlsx (Supplementary material), 172KB
Name:
2262019-Suppl_4.xlsx
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/vnd.openxmlformats-officedocument.spreadsheetml.sheet / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
2262019_Suppl_5.xlsx (Supplementary material), 951KB
Name:
2262019_Suppl_5.xlsx
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/vnd.openxmlformats-officedocument.spreadsheetml.sheet / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
2262019_Suppl_6.xls (Supplementary material), 342KB
Name:
2262019_Suppl_6.xls
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/vnd.ms-excel / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show
hide
Locator:
http://dx.doi.org/10.1038/nature14568 (Publisher version)
Description:
-

Creators

show
hide
 Creators:
Pfender, S., Author
Kuznetsov, V., Author
Pasternak, M., Author
Tischer, T., Author
Santhanam, B., Author
Schuh, M.1, Author              
Affiliations:
1Department of Meiosis, MPI for Biophysical Chemistry, Max Planck Society, ou_2205654              

Content

show
hide
Free keywords: -
 Abstract: During fertilization, an egg and a sperm fuse to form a new embryo. Eggs develop from oocytes in a process called meiosis. Meiosis in human oocytes is highly error-prone, and defective eggs are the leading cause of pregnancy loss and several genetic disorders such as Down's syndrome. Which genes safeguard accurate progression through meiosis is largely unclear. Here we develop high-content phenotypic screening methods for the systematic identification of mammalian meiotic genes. We targeted 774 genes by RNA interference within follicle-enclosed mouse oocytes to block protein expression from an early stage of oocyte development onwards. We then analysed the function of several genes simultaneously by high-resolution imaging of chromosomes and microtubules in live oocytes and scored each oocyte quantitatively for 50 phenotypes, generating a comprehensive resource of meiotic gene function. The screen generated an unprecedented annotated data set of meiotic progression in 2,241 mammalian oocytes, which allowed us to analyse systematically which defects are linked to abnormal chromosome segregation during meiosis, identifying progression into anaphase with misaligned chromosomes as well as defects in spindle organization as risk factors. This study demonstrates how high-content screens can be performed in oocytes, and allows systematic studies of meiosis in mammals.

Details

show
hide
Language(s): eng - English
 Dates: 2015-07-062015-08-13
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1038/nature14568
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Nature
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 524 (7564) Sequence Number: - Start / End Page: 239 - 242 Identifier: -