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  Error-prone chromosome-mediated spindle assembly favors chromosome segregation defects in human oocytes.

Holubcová, Z., Blayney, M., Elder, K., & Schuh, M. (2015). Error-prone chromosome-mediated spindle assembly favors chromosome segregation defects in human oocytes. Science, 348(6239), 1143-1147. doi:10.1126/science.aaa9529.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002A-1B71-6 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002A-2071-1
Genre: Journal Article

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 Creators:
Holubcová, Z., Author
Blayney, M., Author
Elder, K., Author
Schuh, M.1, Author              
Affiliations:
1Department of Meiosis, MPI for Biophysical Chemistry, Max Planck Society, ou_2205654              

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 Abstract: Aneuploidy in human eggs is the leading cause of pregnancy loss and several genetic disorders such as Down syndrome. Most aneuploidy results from chromosome segregation errors during the meiotic divisions of an oocyte, the egg’s progenitor cell. The basis for particularly error-prone chromosome segregation in human oocytes is not known. We analyzed meiosis in more than 100 live human oocytes and identified an error-prone chromosome-mediated spindle assembly mechanism as a major contributor to chromosome segregation defects. Human oocytes assembled a meiotic spindle independently of either centrosomes or other microtubule organizing centers. Instead, spindle assembly was mediated by chromosomes and the small guanosine triphosphatase Ran in a process requiring ~16 hours. This unusually long spindle assembly period was marked by intrinsic spindle instability and abnormal kinetochore-microtubule attachments, which favor chromosome segregation errors and provide a possible explanation for high rates of aneuploidy in human eggs.

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Language(s): eng - English
 Dates: 2015-06-05
 Publication Status: Published online
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 Rev. Method: Peer
 Identifiers: DOI: 10.1126/science.aaa9529
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Title: Science
Source Genre: Journal
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Pages: - Volume / Issue: 348 (6239) Sequence Number: - Start / End Page: 1143 - 1147 Identifier: -