ausblenden:
Schlagwörter:
Ca2?-binding proteins, Sarcoplasmic
reticulum, Animal models, Danio rerio
Zusammenfassung:
Calsequestrin (Casq) is a high capacity, low
affinity Ca2?-binding protein, critical for Ca2?-buffering in
cardiac and skeletal muscle sarcoplasmic reticulum. All
vertebrates have multiple genes encoding for different
Casq isoforms. Increasing interest has been focused on
mammalian and human Casq genes since mutations of both
cardiac (Casq2) and skeletal muscle (Casq1) isoforms
cause different, and sometime severe, human pathologies.
Danio rerio (zebrafish) is a powerful model for studying
function and mutations of human proteins. In this work,
expression, biochemical properties cellular and sub-cellular
localization of D. rerio native Casq isoforms are investigated.
By quantitative PCR, three mRNAs were detected in
skeletal muscle and heart with different abundances. Three
zebrafish Casqs: Casq1a, Casq1b and Casq2 were identified
by mass spectrometry (Data are available via ProteomeXchange
with identifier PXD002455). Skeletal and
cardiac zebrafish calsequestrins share properties with
mammalian Casq1 and Casq2. Skeletal Casqs were found
primarily, but not exclusively, at the sarcomere Z-line level
where terminal cisternae of sarcoplasmic reticulum are
located.
