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  The transition from meiotic to mitotic spindle assembly is gradual during early mammalian development.

Courtois, A., Schuh, M., Ellenberg, J., & Hiiragi, T. (2012). The transition from meiotic to mitotic spindle assembly is gradual during early mammalian development. Journal of Cell Biology, 198(3), 357-370. doi:10.1083/jcb.201202135.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002A-1BA2-5 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002A-205A-8
Genre: Journal Article

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 Creators:
Courtois, A., Author
Schuh, M.1, Author              
Ellenberg, J., Author
Hiiragi, T., Author
Affiliations:
1Department of Meiosis, MPI for Biophysical Chemistry, Max Planck Society, ou_2205654              

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 Abstract: The transition from meiosis to mitosis, classically defined by fertilization, is a fundamental process in development. However, its mechanism remains largely unexplored. In this paper, we report a surprising gradual transition from meiosis to mitosis over the first eight divisions of the mouse embryo. The first cleavages still largely share the mechanism of spindle formation with meiosis, during which the spindle is self-assembled from randomly distributed microtubule-organizing centers (MTOCs) without centrioles, because of the concerted activity of dynein and kinesin-5. During preimplantation development, the number of cellular MTOCs progressively decreased, the spindle pole gradually became more focused, and spindle length progressively scaled down with cell size. The typical mitotic spindle with centrin-, odf2-, kinesin-12–, and CP110-positive centrosomes was established only in the blastocyst. Overall, the transition from meiosis to mitosis progresses gradually throughout the preimplantation stage in the mouse embryo, thus providing a unique system to study the mechanism of centrosome biogenesis in vivo.

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Language(s): eng - English
 Dates: 2012-08
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1083/jcb.201202135
 Degree: -

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Title: Journal of Cell Biology
Source Genre: Journal
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Publ. Info: -
Pages: - Volume / Issue: 198 (3) Sequence Number: - Start / End Page: 357 - 370 Identifier: -