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  The transition from meiotic to mitotic spindle assembly is gradual during early mammalian development

Courtois, A., Schuh, M., Ellenberg, J., & Hiiragi, T. (2012). The transition from meiotic to mitotic spindle assembly is gradual during early mammalian development. Journal of Cell Biology, 198(3), 357-370. doi:10.1083/jcb.201202135.

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Courtois, A., Author
Schuh, M.1, Author           
Ellenberg, J., Author
Hiiragi, T., Author
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1Department of Meiosis, MPI for Biophysical Chemistry, Max Planck Society, Am Faßberg 11, 37077 Göttingen, DE, ou_2205654              

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 Abstract: The transition from meiosis to mitosis, classically defined by fertilization, is a fundamental process in development. However, its mechanism remains largely unexplored. In this paper, we report a surprising gradual transition from meiosis to mitosis over the first eight divisions of the mouse embryo. The first cleavages still largely share the mechanism of spindle formation with meiosis, during which the spindle is self-assembled from randomly distributed microtubule-organizing centers (MTOCs) without centrioles, because of the concerted activity of dynein and kinesin-5. During preimplantation development, the number of cellular MTOCs progressively decreased, the spindle pole gradually became more focused, and spindle length progressively scaled down with cell size. The typical mitotic spindle with centrin-, odf2-, kinesin-12–, and CP110-positive centrosomes was established only in the blastocyst. Overall, the transition from meiosis to mitosis progresses gradually throughout the preimplantation stage in the mouse embryo, thus providing a unique system to study the mechanism of centrosome biogenesis in vivo.

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Language(s): eng - English
 Dates: 2012-08
 Publication Status: Issued
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 Rev. Type: Peer
 Identifiers: DOI: 10.1083/jcb.201202135
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Title: Journal of Cell Biology
Source Genre: Journal
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Publ. Info: New York, NY : Rockefeller Institute Press
Pages: - Volume / Issue: 198 (3) Sequence Number: - Start / End Page: 357 - 370 Identifier: ISSN: 0021-9525
CoNE: https://pure.mpg.de/cone/journals/resource/991042742946024_1