English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Spire-type actin nucleators cooperate with formin-2 to drive asymmetric oocyte division.

Pfender, S., Kuznetsov, V., Pleiser, S., Kerkhoff, E., & Schuh, M. (2011). Spire-type actin nucleators cooperate with formin-2 to drive asymmetric oocyte division. Current Biology, 21(11), 955-960. doi:10.1016/j.cub.2011.04.029.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002A-22F5-7 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002A-22F9-0
Genre: Journal Article

Files

show Files
hide Files
:
2262699.pdf (Publisher version), 2MB
Name:
2262699.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
:
2262699_Suppl.pdf (Supplementary material), 930KB
Name:
2262699_Suppl.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show
hide
Description:
-

Creators

show
hide
 Creators:
Pfender, S., Author
Kuznetsov, V., Author
Pleiser, S., Author
Kerkhoff, E., Author
Schuh, M.1, Author              
Affiliations:
1Department of Meiosis, MPI for Biophysical Chemistry, Max Planck Society, ou_2205654              

Content

show
hide
Free keywords: -
 Abstract: Oocytes mature into eggs by extruding half of their chromosomes in a small cell termed the polar body. Asymmetric oocyte division is essential for fertility [1], but despite its importance, little is known about its mechanism. In mammals, the meiotic spindle initially forms close to the center of the oocyte. Thus, two steps are required for asymmetric meiotic division: first, asymmetric spindle positioning and second, polar body extrusion. Here, we identify Spire1 and Spire2 as new key factors in asymmetric division of mouse oocytes. Spire proteins are novel types of actin nucleators that drive nucleation of actin filaments with their four WH2 actin-binding domains [2, 3, 4, 5 and 6]. We show that Spire1 and Spire2 first mediate asymmetric spindle positioning by assembling an actin network that serves as a substrate for spindle movement. Second, they drive polar body extrusion by promoting assembly of the cleavage furrow. Our data suggest that Spire1 and Spire2 cooperate with Formin-2 (Fmn2) to nucleate actin filaments in mouse oocytes and that both types of nucleators act as a functional unit. This study not only reveals how Spire1 and Spire2 drive two critical steps of asymmetric oocyte division, but it also uncovers the first physiological function of Spire-type actin nucleators in vertebrates.

Details

show
hide
Language(s): eng - English
 Dates: 2011-06
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1016/j.cub.2011.04.029
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Current Biology
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 21 (11) Sequence Number: - Start / End Page: 955 - 960 Identifier: -