English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Incorporation of Drosophila CID/CENP-A and CENP-C into centromeres during early embryonic anaphase.

Schuh, M., Lehner, C. F., & Heidmann, S. (2007). Incorporation of Drosophila CID/CENP-A and CENP-C into centromeres during early embryonic anaphase. Current Biology, 17(3), 237-243. doi:10.1016/j.cub.2006.11.051.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002A-23E4-7 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002A-23E8-0
Genre: Journal Article

Files

show Files
hide Files
:
2262765.pdf (Publisher version), 2MB
Name:
2262765.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
2262765_Suppl.pdf (Supplementary material), 869KB
Name:
2262765_Suppl.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show
hide
Description:
-

Creators

show
hide
 Creators:
Schuh, M.1, Author              
Lehner, C. F., Author
Heidmann, S., Author
Affiliations:
1Department of Meiosis, MPI for Biophysical Chemistry, Max Planck Society, ou_2205654              

Content

show
hide
Free keywords: DNA
 Abstract: The centromere/kinetochore complex is indispensable for accurate segregation of chromosomes during cell divisions when it serves as the attachment site for spindle microtubules. Centromere identity in metazoans is believed to be governed by epigenetic mechanisms, because the highly repetitive centromeric DNA is neither sufficient nor required for specifying the assembly site of the kinetochore 1, 2, 3 and 4. A candidate for an epigenetic mark is the centromere-specific histone H3 variant CENP-A that replaces H3 in alternating blocks of chromatin exclusively in active centromeres 1, 2, 5 and 6. CENP-A acts as an initiator of kinetochore assembly, but the detailed dynamics of the deposition of metazoan CENP-A and of other constitutive kinetochore components are largely unknown 1, 2, 7, 8, 9 and 10. Here we show by quantitative fluorescence measurements in living early embryos that functional fluorescent fusion proteins of the Drosophila CENP-A and CENP-C homologs are rapidly incorporated into centromeres during anaphase. This incorporation is independent of ongoing DNA synthesis and pulling forces generated by the mitotic spindle, but strictly coupled to mitotic progression. Thus, our findings uncover a strikingly dynamic behavior of centromere components in anaphase.

Details

show
hide
Language(s): eng - English
 Dates: 2007-01-112007-02-06
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1016/j.cub.2006.11.051
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Current Biology
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 17 (3) Sequence Number: - Start / End Page: 237 - 243 Identifier: -