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  Self-organization of MTOCs replaces centrosome function during acentrosomal spindle assembly in live mouse oocytes.

Schuh, M., & Ellenberg, J. (2007). Self-organization of MTOCs replaces centrosome function during acentrosomal spindle assembly in live mouse oocytes. Cell, 130(3), 484-498. doi:10.1016/j.cell.2007.06.025.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002A-242A-3 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002A-2438-3
Genre: Journal Article

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 Creators:
Schuh, M.1, Author              
Ellenberg, J., Author
Affiliations:
1Department of Meiosis, MPI for Biophysical Chemistry, Max Planck Society, ou_2205654              

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Free keywords: CELLBIO
 Abstract: Chromosome segregation in mammalian oocytes is driven by a microtubule spindle lacking centrosomes. Here, we analyze centrosome-independent spindle assembly by quantitative high-resolution confocal imaging in live maturing mouse oocytes. We show that spindle assembly proceeds by the self-organization of over 80 microtubule organizing centers (MTOCs) that form de novo from a cytoplasmic microtubule network in prophase and that functionally replace centrosomes. Initially distributed throughout the ooplasm, MTOCs congress at the center of the oocyte, where they contribute to a massive, Ran-dependent increase of the number of microtubules after nuclear envelope breakdown and to the individualization of clustered chromosomes. Through progressive MTOC clustering and activation of kinesin-5, the multipolar MTOC aggregate self-organizes into a bipolar intermediate, which then elongates and thereby establishes chromosome biorientation. Finally, a stable barrel-shaped acentrosomal metaphase spindle with oscillating chromosomes and astral-like microtubules forms that surprisingly exhibits key properties of a centrosomal spindle.

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Language(s): eng - English
 Dates: 2007-08-092007-08-10
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1016/j.cell.2007.06.025
 Degree: -

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Title: Cell
Source Genre: Journal
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Publ. Info: -
Pages: - Volume / Issue: 130 (3) Sequence Number: - Start / End Page: 484 - 498 Identifier: -