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  Resolution of chiasmata in oocytes requires separase-mediated proteolysis.

Kudo, N. R., Wassmann, K., Anger, M., Schuh, M., Wirth, K. G., Xu, H., et al. (2006). Resolution of chiasmata in oocytes requires separase-mediated proteolysis. Cell, 126(1), 135-146. doi:10.1016/j.cell.2006.05.033.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002A-24C9-E Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002A-24D0-B
Genre: Journal Article

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 Creators:
Kudo , N. R., Author
Wassmann , K., Author
Anger , M., Author
Schuh, M.1, Author              
Wirth, K. G., Author
Xu , H., Author
Helmhart, W., Author
Kudo, H., Author
Mckay , M., Author
Maro , B., Author
Ellenberg , J., Author
de Boer , P., Author
Nasmyth , K., Author
Affiliations:
1Department of Meiosis, MPI for Biophysical Chemistry, Max Planck Society, ou_2205654              

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 Abstract: n yeast, resolution of chiasmata in meiosis I requires proteolytic cleavage along chromosome arms of cohesin's Rec8 subunit by separase. Since activation of separase by the anaphase-promoting complex (APC/C) is supposedly not required for meiosis I in Xenopus oocytes, it has been suggested that animal cells might resolve chiasmata by a separase-independent mechanism related to the so-called “prophase pathway” that removes cohesin from chromosome arms during mitosis. By expressing Cre recombinase from a zona pellucida promoter, we have deleted a floxed allele of separase specifically in mouse oocytes. This prevents removal of Rec8 from chromosome arms and resolution of chiasmata. It also hinders extrusion of the first polar body (PBE) and causes female sterility. mRNA encoding wild-type but not catalytically inactive separase restores chiasma resolution. Both types of mRNA restore PBE. Proteolytic activity of separase is therefore essential for Rec8's removal from chromosome arms and for chiasma resolution but not for PBE.

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Language(s): eng - English
 Dates: 2006-07-132006-07-14
 Publication Status: Published in print
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 Rev. Method: Peer
 Identifiers: DOI: 10.1016/j.cell.2006.05.033
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Title: Cell
Source Genre: Journal
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Pages: - Volume / Issue: 126 (1) Sequence Number: - Start / End Page: 135 - 146 Identifier: -