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  Functional fluorescent Ca2+ indicator proteins in transgenic mice under TET control.

Hasan, M. T., Friedrich, R. W., Euler, T., Larkum, M. E., Giese, G., Both, M., et al. (2004). Functional fluorescent Ca2+ indicator proteins in transgenic mice under TET control. PLoS Biology, 2(6): e163, pp. 763-775. doi:10.1371/journal.pbio.0020163.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002A-26D8-7 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002A-26D9-5
Genre: Journal Article
Alternative Title : Functional fluorescent Ca2+ indicator proteins in transgenic mice under TET control.

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 Creators:
Hasan, Mazahir T.1, 2, Author              
Friedrich, Rainer W.2, Author              
Euler, Thomas2, Author              
Larkum, Matthew E.3, Author              
Giese, Günter2, Author              
Both, Matthias2, Author              
Dübel, Jens2, Author              
Waters, David Jack3, Author              
Bujard, Hermann, Author
Griesbeck, Oliver, Author
Tsien, Roger Y., Author
Nagai, Takeharu, Author
Miyawaki, Atsushi, Author
Denk, Winfried2, Author              
Affiliations:
1Department of Molecular Neurobiology, Max Planck Institute for Medical Research, Max Planck Society, ou_1497704              
2Department of Biomedical Optics, Max Planck Institute for Medical Research, Max Planck Society, ou_1497699              
3Department of Cell Physiology, Max Planck Institute for Medical Research, Max Planck Society, ou_1497701              

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 Abstract: Genetically encoded fluorescent calcium indicator proteins (FCIPs) are promising tools to study calcium dynamics in many activity-dependent molecular and cellular processes. Great hopes-for the measurement of population activity, in particular-have therefore been placed on calcium indicators derived from the green fluorescent protein and their expression in (selected) neuronal populations. Calcium transients can rise within milliseconds, making them suitable as reporters of fast neuronal activity. We here report the production of stable transgenic mouse lines with two different functional calcium indicators, inverse pericam and camgaroo-2, under the control of the tetracycline-inducible promoter. Using a variety of in vitro and in vivo assays, we find that stimuli known to increase intracellular calcium concentration (somatically triggered action potentials (APs) and synaptic and sensory stimulation) can cause substantial and rapid changes in FCIP fluorescence of inverse pericam and camgaroo-2.

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Language(s): eng - English
 Dates: 2003-09-162004-04-062004-06-152004-06-01
 Publication Status: Published in print
 Pages: 13
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Degree: -

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Title: PLoS Biology
  Other : PLoS Biol.
Source Genre: Journal
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Publ. Info: Public Library of Science
Pages: - Volume / Issue: 2 (6) Sequence Number: e163 Start / End Page: 763 - 775 Identifier: ISSN: 1544-9173
CoNE: https://pure.mpg.de/cone/journals/resource/111056649444170