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  Travelling-wave ion mobility and negative ion fragmentation of high-mannose N-glycans

Harvey, D. J., Scarff, C. A., Edgeworth, M., Struwe, W. B., Pagel, K., Thalassinos, K., et al. (2016). Travelling-wave ion mobility and negative ion fragmentation of high-mannose N-glycans. Journal of Mass Spectrometry, 51(3), 219-235. doi:10.1002/jms.3738.

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Harvey, David J.1, 2, Autor
Scarff, Charlotte A. 2, 3, Autor
Edgeworth, Matthew 2, Autor
Struwe, Weston B.1, 4, Autor
Pagel, Kevin5, 6, Autor           
Thalassinos, Konstantinos7, 8, Autor
Crispin, Max 1, Autor
Scrivens, Jim2, Autor
Affiliations:
1Oxford Glycobiology Institute, Department of Biochemistry, University of Oxford, South Park Road, Oxford OX1 3QU, UK, ou_persistent22              
2Department of Biological Sciences, University of Warwick, Coventry CV47AL, UK, ou_persistent22              
3Astbury Centre for Structural Molecular Biology, School of Molecular and Cellular Biology, University of Leeds, Leeds LS2 9JT, UK, ou_persistent22              
4Chemistry Research Laboratory, 12 Mansfield Road, OX1 3TA, UK, ou_persistent22              
5Molecular Physics, Fritz Haber Institute, Max Planck Society, ou_634545              
6Institute of Chemistry and Biochemistry, Freie Universität Berlin, Berlin, Germany, ou_persistent22              
7Institute of Structural and Molecular Biology, Division of Biosciences, University College London, London WC1E 6BT, UK, ou_persistent22              
8Institute of Structural and Molecular Biology, Department of Biological Sciences, Birkbeck College, University of London, London, UK, ou_persistent22              

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 Zusammenfassung: The isomeric structure of high-mannose N-glycans can significantly impact biological recognition events. Here, the utility of travelling-wave ion mobility mass spectrometry for isomer separation of high-mannose N-glycans is investigated. Negative ion fragmentation using collision-induced dissociation gave more informative spectra than positive ion spectra with mass-different fragment ions characterizing many of the isomers. Isomer separation by ion mobility in both ionization modes was generally limited, with the arrival time distributions (ATD) often showing little sign of isomers. However, isomers could be partially resolved by plotting extracted fragment ATDs of the diagnostic fragment ions from the negative ion spectra, and the fragmentation spectra of the isomers could be extracted by using ions from limited areas of the ATD peak. In some cases, asymmetric ATDs were observed, but no isomers could be detected by fragmentation. In these cases, it was assumed that conformers or anomers were being separated. Collision cross sections of the isomers in positive and negative fragmentation mode were estimated from travelling-wave ion mobility mass spectrometry data using dextran glycans as calibrant. More complete collision cross section data were achieved in negative ion mode by utilizing the diagnostic fragment ions. Examples of isomer separations are shown for N-glycans released from the well-characterized glycoproteins chicken ovalbumin, porcine thyroglobulin and gp120 from the human immunodeficiency virus. In addition to the cross-sectional data, details of the negative ion collision-induced dissociation spectra of all resolved isomers are discussed.

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 Datum: 2015-12-012015-09-212015-12-022016-03-042016-03-09
 Publikationsstatus: Erschienen
 Seiten: 17
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: Expertenbegutachtung
 Identifikatoren: DOI: 10.1002/jms.3738
 Art des Abschluß: -

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Titel: Journal of Mass Spectrometry
Genre der Quelle: Zeitschrift
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Ort, Verlag, Ausgabe: Chichester, England : Wiley
Seiten: 17 Band / Heft: 51 (3) Artikelnummer: - Start- / Endseite: 219 - 235 Identifikator: ISSN: 1076-5174
CoNE: https://pure.mpg.de/cone/journals/resource/954926950786