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  Sulforhodamine 101 as a specific marker of astroglia in the neocortex in vivo

Nimmerjahn, A., Kirchhoff, F., Kerr, J., & Helmchen, F. (2004). Sulforhodamine 101 as a specific marker of astroglia in the neocortex in vivo. Nature methods, 1(1), 31-37. doi:10.1038/nmeth706.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002A-33F2-8 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002A-33F3-6
Genre: Journal Article
Alternative Title : Sulforhodamine 101 as a specific marker of astroglia in the neocortex in vivo

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Nimmerjahn, Axel1, Author              
Kirchhoff, Frank, Author
Kerr, Jason1, 2, Author              
Helmchen, Fritjof1, Author              
Affiliations:
1Department of Cell Physiology, Max Planck Institute for Medical Research, Max Planck Society, ou_1497701              
2Department of Biomedical Optics, Max Planck Institute for Medical Research, Max Planck Society, ou_1497699              

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 Abstract: Glial cells have been identified as key signaling components in the brain; however, methods to investigate their structure and function in vivo have been lacking. Here, we describe a new, highly selective approach for labeling astrocytes in intact rodent neocortex that allows in vivo imaging using two-photon microscopy. The red fluorescent dye sulforhodamine 101 (SR101) was specifically taken up by protoplasmic astrocytes after brief exposure to the brain surface. Specificity was confirmed by immunohistochemistry. In addition, SR101 labeled enhanced green fluorescent protein (EGFP)-expressing astrocytes but not microglial cells in transgenic mice. We used SR101 labeling to quantify morphological characteristics of astrocytes and to visualize their close association with the cortical microvasculature. Furthermore, by combining this method with calcium indicator loading of cell populations, we demonstrated distinct calcium dynamics in astroglial and neuronal networks. We expect SR101 staining to become a principal tool for investigating astroglia in vivo.

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Language(s): eng - English
 Dates: 2004-06-022004-08-262004-09-292004-10-01
 Publication Status: Published in print
 Pages: 6
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
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Title: Nature methods
  Other : Nature methods
Source Genre: Journal
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Publ. Info: New York, NY : Nature Pub. Group
Pages: - Volume / Issue: 1 (1) Sequence Number: - Start / End Page: 31 - 37 Identifier: ISSN: 1548-7091
CoNE: https://pure.mpg.de/cone/journals/resource/111088195279556