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  Breaking the diffraction limit of light-sheet fluorescence microscopy by RESOLFT.

Hoyer, P., de Medeiros, G., Balazs, B., Norlin, N., Besir, C., Hanne, J., et al. (2016). Breaking the diffraction limit of light-sheet fluorescence microscopy by RESOLFT. Proceedings of the National Academy of Sciences of the United States of America, 113(13), 3442-3446. doi: 10.1073/pnas.1522292113.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002A-35BB-1 Version Permalink: http://hdl.handle.net/21.11116/0000-0002-A710-F
Genre: Journal Article

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 Creators:
Hoyer, P., Author
de Medeiros, G., Author
Balazs, B., Author
Norlin, N., Author
Besir, C., Author
Hanne, J., Author
Kräusslich, H. G., Author
Engelhardt, J., Author
Sahl, S. J.1, Author              
Hell, S. W.1, Author              
Hufnagel, L., Author
Affiliations:
1Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society, ou_578627              

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Free keywords: light-sheet microscopy; RESOLFT; optical nanoscopy; 3D; live-cell imaging
 Abstract: We present a plane-scanning RESOLFT [reversible saturable/switchable optical (fluorescence) transitions] light-sheet (LS) nanoscope, which fundamentally overcomes the diffraction barrier in the axial direction via confinement of the fluorescent molecular state to a sheet of subdiffraction thickness around the focal plane. To this end, reversibly switchable fluorophores located right above and below the focal plane are transferred to a nonfluorescent state at each scanning step. LS-RESOLFT nanoscopy offers wide-field 3D imaging of living biological specimens with low light dose and axial resolution far beyond the diffraction barrier. We demonstrate optical sections that are thinner by 5–12-fold compared with their conventional diffraction-limited LS analogs.

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Language(s): eng - English
 Dates: 2016-03-29
 Publication Status: Published online
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 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1073/pnas.1522292113
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Title: Proceedings of the National Academy of Sciences of the United States of America
Source Genre: Journal
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Pages: - Volume / Issue: 113 (13) Sequence Number: - Start / End Page: 3442 - 3446 Identifier: -