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  Parallelized ultra-high throughput microfluidic emulsifier for multiplex kinetic assays

Lim, J., Caen, O., Vrignon, J., Konrad, M., Taly, V., & Baret, J.-C. (2015). Parallelized ultra-high throughput microfluidic emulsifier for multiplex kinetic assays. Biomicrofluidics, 9(3): 034101. doi:10.1063/1.4919415.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002A-3997-F Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002A-E188-3
Genre: Journal Article

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 Creators:
Lim, Jiseok1, Author              
Caen, O., Author
Vrignon, J., Author
Konrad, M., Author
Taly, V., Author
Baret, Jean-Christophe1, Author              
Affiliations:
1Max Planck Research Group Droplets, Membranes and Interfaces, Max Planck Institute for Dynamics and Self-Organization, Max Planck Society, ou_2063290              

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Free keywords: Fluid drops; Emulsions; Microscale flows; Fluidic devices; Poiseuille flow
 Abstract: Droplet-based microfluidic technologies are powerful tools for applications requiring high-throughput, for example, in biochemistry or material sciences. Several systems have been proposed for the high-throughput production of monodisperse emulsions by parallelizing multiple droplet makers. However, these systems have two main limitations: (1) they allow the use of only a single disperse phase; (2) they are based on multiple layer microfabrication techniques. We present here a pipette-and-play solution offering the possibility of manipulating simultaneously 10 different disperse phases on a single layer device. This system allows high-throughput emulsion production using aqueous flow rates of up to 26 ml/h (>110 000 drops/s) leading to emulsions with user-defined complex chemical composition. We demonstrate the multiplex capabilities of our system by measuring the kinetics of β-galactosidase in droplets using nine different concentrations of a fluorogenic substrate.

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Language(s): eng - English
 Dates: 2015-05-052015-05
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: BibTex Citekey: lim_biofl_2015
DOI: 10.1063/1.4919415
 Degree: -

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Title: Biomicrofluidics
Source Genre: Journal
 Creator(s):
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Publ. Info: -
Pages: 11 Volume / Issue: 9 (3) Sequence Number: 034101 Start / End Page: - Identifier: -