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  Gemini encodes a zebrafish L-type calcium channel that localizes at sensory hair cell ribbon synapses

Sidi, S., Busch-Nentwich, E., Friedrich, R. W., Schoenberger, U., & Nicolson, T. (2004). Gemini encodes a zebrafish L-type calcium channel that localizes at sensory hair cell ribbon synapses. The Journal of Neuroscience: the Official Journal of the Society for Neuroscience, 24(17), 4213-4223. doi:10.1523/JNEUROSCI.0223-04.2004.

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Alternative Title : Gemini encodes a zebrafish L-type calcium channel that localizes at sensory hair cell ribbon synapses

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 Creators:
Sidi, Samuel, Author
Busch-Nentwich, Elisabeth, Author
Friedrich, Rainer W.1, Author           
Schoenberger, Ulrike, Author
Nicolson, Teresa, Author
Affiliations:
1Department of Biomedical Optics, Max Planck Institute for Medical Research, Max Planck Society, ou_1497699              

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Free keywords: gemini; L-type calcium channels; deafness; hair cell; ribbon synapse; microphonics; synaptic transmission
 Abstract: L-type Ca2+ channels (LTCCs) drive the bulk of voltage-gated Ca2+ entry in vertebrate inner ear hair cells (HCs) and are essential for mammalian auditory processing. LTCC currents have been implicated in neurotransmitter release at the HC afferent active zone, the ribbon synapse. It is likely that LTCCs play a direct role in vesicle fusion; however, the subcellular localization of the channels in HCs has not been fully resolved. Via positional cloning, we show that mutations in a zebrafish LTCC encoding gene, cav1.3a, underlie the auditory-vestibular defects of gemini (gem) circler mutants. gem homozygous receptor mutant HCs display normal cell viability, afferent synaptogenesis, and peripheral innervation, yet exhibit strongly reduced extracellular potentials (approximately 50% of wild-type potentials). Apical FM1-43 uptake, however, is unaffected in gem mutant HCs, suggesting that mechanotransduction channels are functional. Using a Gem-specific antibody, we show that the bulk of Gem/Ca(v)1.3a immunoreactivity in HCs is restricted to basally located focal spots. The number and location of focal spots relative to nerve terminals, and their remarkable ring-shaped structure, which is reminiscent of synaptic dense bodies, are consistent with Gem/Ca(v)1.3a channels clustering at HC ribbon synapses.

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Language(s): eng - English
 Dates: 2004-03-122003-11-212004-03-152004-04-28
 Publication Status: Issued
 Pages: 10
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
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Title: The Journal of Neuroscience : the Official Journal of the Society for Neuroscience
  Other : J. Neurosci.
Source Genre: Journal
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Publ. Info: Baltimore, MD : The Society
Pages: - Volume / Issue: 24 (17) Sequence Number: - Start / End Page: 4213 - 4223 Identifier: ISSN: 0270-6474
CoNE: https://pure.mpg.de/cone/journals/resource/954925502187_1