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  Multivalent display of minimal Clostridium difficile glycan epitopes mimics antigenic properties of larger glycans

Broecker, F., Hanske, J., Martin, C. E., Baek, J. Y., Wahlbrink, A., Wojcik, F., et al. (2016). Multivalent display of minimal Clostridium difficile glycan epitopes mimics antigenic properties of larger glycans. Nature Communications, 7: 11224. doi:10.1038/ncomms11224.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002A-41A9-E Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002A-C6F7-A
Genre: Journal Article

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 Creators:
Broecker, Felix1, Author              
Hanske, Jonas2, Author              
Martin, Christopher E.3, Author              
Baek, Ju Yuel3, Author              
Wahlbrink, Annette4, Author              
Wojcik, Felix5, Author              
Hartmann, Laura5, Author              
Rademacher, Christoph2, Author              
Chakkumkal, Anish1, Author              
Seeberger, Peter H.6, Author              
Affiliations:
1Chakkumal Anish, Biomolekulare Systeme, Max Planck Institute of Colloids and Interfaces, Max Planck Society, ou_1863299              
2Christoph Rademacher, Biomolekulare Systeme, Max Planck Institute of Colloids and Interfaces, Max Planck Society, ou_1863300              
3Peter H. Seeberger - Vaccine Development, Biomolekulare Systeme, Max Planck Institute of Colloids and Interfaces, Max Planck Society, ou_1863308              
4Biomolekulare Systeme, Max Planck Institute of Colloids and Interfaces, Max Planck Society, ou_1863286              
5Laura Hartmann, Biomolekulare Systeme, Max Planck Institute of Colloids and Interfaces, Max Planck Society, ou_1863305              
6Peter H. Seeberger, Biomolekulare Systeme, Max Planck Institute of Colloids and Interfaces, Max Planck Society, ou_2040285              

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Free keywords: Open Access
 Abstract: Synthetic cell-surface glycans are promising vaccine candidates against Clostridium difficile. The complexity of large, highly antigenic and immunogenic glycans is a synthetic challenge. Less complex antigens providing similar immune responses are desirable for vaccine development. Based on molecular-level glycan-antibody interaction analyses, we here demonstrate that the C. difficile surface polysaccharide-I (PS-I) can be resembled by multivalent display of minimal disaccharide epitopes on a synthetic scaffold that does not participate in binding. We show that antibody avidity as a measure of antigenicity increases by about five orders of magnitude when disaccharides are compared with constructs containing five disaccharides. The synthetic, pentavalent vaccine candidate containing a peptide T-cell epitope elicits weak but highly specific antibody responses to larger PS-I glycans in mice. This study highlights the potential of multivalently displaying small oligosaccharides to achieve antigenicity characteristic of larger glycans. The approach may result in more cost-efficient carbohydrate vaccines with reduced synthetic effort.

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 Dates: 2016-04-19
 Publication Status: Published in print
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 Rev. Method: -
 Identifiers: DOI: 10.1038/ncomms11224
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Title: Nature Communications
  Abbreviation : Nat. Commun.
Source Genre: Journal
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Publ. Info: London : Nature Publishing Group
Pages: - Volume / Issue: 7 Sequence Number: 11224 Start / End Page: - Identifier: ISSN: 2041-1723