English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Functional identification of primate lateral geniculate nucleus projections to visual cortex using optogenetics and electrical stimulation

Klein, C., Evrard, H., Shapcott, K., Haverkamp, S., Logothetis, N., & Schmid, M. (2015). Functional identification of primate lateral geniculate nucleus projections to visual cortex using optogenetics and electrical stimulation. Poster presented at 45th Annual Meeting of the Society for Neuroscience (Neuroscience 2015), Chicago, IL, USA.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002A-43E3-3 Version Permalink: http://hdl.handle.net/21.11116/0000-0000-F80E-A
Genre: Poster

Files

show Files

Locators

show
hide
Locator:
http://www.sfn.org/am2015/ (Publisher version)
Description:
-

Creators

show
hide
 Creators:
Klein, C1, Author              
Evrard, HC1, 2, Author              
Shapcott, K, Author
Haverkamp, S, Author
Logothetis, NK1, Author              
Schmid, MC, Author
Affiliations:
1Department Physiology of Cognitive Processes, Max Planck Institute for Biological Cybernetics, Max Planck Society, ou_1497798              
2Dept. Empirical Inference, Max Planck Institute for Intelligent System, Max Planck Society, ou_1497647              

Content

show
hide
Free keywords: -
 Abstract: Optogenetics and electrical stimulation are routinely used to assess neuronal connectivity. However cell-specific approaches, especially in primates, are still very limited. Here we compare the capacities of optogenetics and electrical stimulation to isolate the specific pathways from the lateral geniculate nucleus (LGN) to primary visual cortex (V1) in the macaque visual system. The organization of LGN into three anatomically separate and neurochemically distinct cell projection systems with virtually no cross-talk provides unique conditions to test for cell-specific targeting by electrical and optogenetic stimulation techniques. For the optogenetics experiments, we injected AAV5-CamKIIα-ChR2-eYFP into the LGN of four macaque monkeys. Histological analysis revealed primarily the predicted laminar expression pattern of the optogenetic construct in CamKIIα-rich LGN konio layers, but also showed some expression in parvalbumin positive magno- and parvo cells. We also observed a retrograde tracing mechanism of the AAV5 virus particles that labeled V1 layer 6 cortico-thalamic feedback neurons and retinal ganglion cells. That expression of the construct also allowed modulation of spiking activity in LGN was confirmed in prior electrophysiology experiments. Neurons expressing ChR2 could be identified reliably based on their short latency (<5ms) spiking responses to direct blue light (473nm) stimulation. Parallel laminar-resolved recordings of the V1 local field potential showed that selective activation of LGN konio layers with optogenetics caused selective electrical current inflow in the supra-granular layers of V1 in agreement with anatomical predictions about the koniocellular projection. Electrical stimulation of LGN konio layers revealed the same supra-granular V1 activation pattern. In contrast, electrical stimulation of LGN parvo layers activated also V1 granular layers in a way that closely resembled visual stimulus driven responses. These findings indicate comparable capacities of both stimulation methods to isolate and identify spatially segregated thalamo-cortical circuit mechanisms of the primate brain.

Details

show
hide
Language(s):
 Dates: 2015-10-19
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: -
 Identifiers: BibTex Citekey: KleinESHLS2015
 Degree: -

Event

show
hide
Title: 45th Annual Meeting of the Society for Neuroscience (Neuroscience 2015)
Place of Event: Chicago, IL, USA
Start-/End Date: -

Legal Case

show

Project information

show

Source 1

show
hide
Title: 45th Annual Meeting of the Society for Neuroscience (Neuroscience 2015)
Source Genre: Proceedings
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: - Sequence Number: 449.09 Start / End Page: - Identifier: -