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  Two-photon imaging of neuronal populations in the primary visual cortex representation of the overhead visual field

Rulla, S., Ng, B., Macke, J., Wallace, D., Sawinski, J., & Kerr, J. (2015). Two-photon imaging of neuronal populations in the primary visual cortex representation of the overhead visual field. Poster presented at 45th Annual Meeting of the Society for Neuroscience (Neuroscience 2015), Chicago, IL, USA.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002A-43F8-6 Version Permalink: http://hdl.handle.net/21.11116/0000-0000-FA8B-A
Genre: Poster

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http://www.sfn.org/am2015/ (Publisher version)
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 Creators:
Rulla, S1, Author              
Ng, B2, Author              
Macke, J3, Author              
Wallace, D1, Author              
Sawinski, J1, Author              
Kerr, J1, Author              
Affiliations:
1Research Group Neural Population Imaging, Max Planck Institute for Biological Cybernetics, Max Planck Society, ou_1497807              
2Research Group Physiology of Sensory Integration, Max Planck Institute for Biological Cybernetics, Max Planck Society, ou_1497808              
3Research Group Computational Vision and Neuroscience, Max Planck Institute for Biological Cybernetics, Max Planck Society, ou_1497805              

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 Abstract: Rodents have a large binocular field of view that extends from the snout to over the animals head. Recent experiments have shown that rodents have a strong, innate, evasive behavior evoked exclusively by stimuli presented above them. However, little is known about the functional properties of cortical neurons that represent the overhead visual field. Here we describe a method for allowing direct optical recording from populations of neurons representing the overhead visual field. Firstly, the conventional microscope objective has been replaced with a periscope coupled to a miniature objective to facilitate placement of a stimulus monitor above the rat’s head. Secondly, we developed a method for presentation of visual stimuli on the OLED display of a tablet running the Android OS, and a camera-based method for calibrating the position of the stimulus display in relation to the animals head. Using this setup, we recorded in rats the activity of neurons in the representation of the overhead visual field of the primary visual cortex in response to a range of stimuli. Neurons were labeled with the calcium indicator OGB-1 with counterstaining of astrocytes using sulforhodamine 101. Stimuli were either an expanding or contracting looming dot, or a moving dot that moved at constant speed along multiple trajectories to cover all positions within the display. In both stimulus types, differing sets of foreground/background luminance were used. Preliminary results show that 19 of the neurons responded with clear and reproducible transients to the looming dot stimulus, and 30 were responsive to moving dot stimuli. The response profiles of neurons to different stimulus types and parameters were further analyzed in detail and compared between cortical areas and receptive field properties established for this cortical region.

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 Dates: 2015-10-18
 Publication Status: Published in print
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 Identifiers: BibTex Citekey: RullaNMWSK2015
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Title: 45th Annual Meeting of the Society for Neuroscience (Neuroscience 2015)
Place of Event: Chicago, IL, USA
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Title: 45th Annual Meeting of the Society for Neuroscience (Neuroscience 2015)
Source Genre: Proceedings
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Pages: - Volume / Issue: - Sequence Number: 232.10 Start / End Page: - Identifier: -