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  Ultrasmall Nanoplatforms as Calcium-Responsive Contrast Agents for Magnetic Resonance Imaging

Moussaron, A., Vibhute, S., Bianchi, A., Gündüz, S., Kolb, S., Sancey, L., et al. (2015). Ultrasmall Nanoplatforms as Calcium-Responsive Contrast Agents for Magnetic Resonance Imaging. Small, 11(37), 4900-4909. doi:10.1002/smll.201500312.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002A-4420-2 Version Permalink: http://hdl.handle.net/21.11116/0000-0000-FE1D-3
Genre: Journal Article

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Moussaron, A, Author
Vibhute, S1, 2, Author              
Bianchi, A, Author
Gündüz, S2, Author              
Kolb, S, Author
Sancey, L, Author
Motto-Ros, V, Author
Rizzitelli, S, Author
Crémillieux, Y, Author
Lux, F, Author
Logothetis, NK1, 2, Author              
Tillement, O, Author
Angelovski, G2, 3, Author              
Affiliations:
1Department Physiology of Cognitive Processes, Max Planck Institute for Biological Cybernetics, Max Planck Society, ou_1497798              
2Max Planck Institute for Biological Cybernetics, Max Planck Society, ou_1497794              
3Research Group MR Neuroimaging Agents, Max Planck Institute for Biological Cybernetics, Max Planck Society, Spemannstrasse 38, 72076 Tübingen, DE, ou_2528691              

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 Abstract: The preparation of ultrasmall and rigid platforms (USRPs) that are covalently coupled to macrocycle-based, calcium-responsive/smart contrast agents (SCAs), and the initial in vitro and in vivo validation of the resulting nanosized probes (SCA-USRPs) by means of magnetic resonance imaging (MRI) is reported. The synthetic procedure is robust, allowing preparation of the SCA-USRPs on a multigram scale. The resulting platforms display the desired MRI activity—i.e., longitudinal relaxivity increases almost twice at 7 T magnetic field strength upon saturation with Ca2+. Cell viability is probed with the MTT assay using HEK-293 cells, which show good tolerance for lower contrast agent concentrations over longer periods of time. On intravenous administration of SCA-USRPs in living mice, MRI studies indicate their rapid accumulation in the renal pelvis and parenchyma. Importantly, the MRI signal increases in both kidney compartments when CaCl2 is also administrated. Laser-induced breakdown spectroscopy experiments confirm accumulation of SCA-USRPs in the renal cortex. To the best of our knowledge, these are the first studies which demonstrate calcium-sensitive MRI signal changes in vivo. Continuing contrast agent and MRI protocol optimizations should lead to wider application of these responsive probes and development of superior functional methods for monitoring calcium-dependent physiological and pathological processes in a dynamic manner.

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 Dates: 2015-10
 Publication Status: Published in print
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 Identifiers: DOI: 10.1002/smll.201500312
BibTex Citekey: MoussaronVBGKSMRCLLTA2015
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Title: Small
Source Genre: Journal
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Pages: - Volume / Issue: 11 (37) Sequence Number: - Start / End Page: 4900 - 4909 Identifier: -