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  An anterograde rabies virus vector for high-resolution large-scale reconstruction of 3D neuron morphology

Haberl, M., Viana da Silva, S., Guest, J., Ginger, M., Ghanem, A., Mulle, C., et al. (2015). An anterograde rabies virus vector for high-resolution large-scale reconstruction of 3D neuron morphology. Brain Structure and Function, 220(3), 1369-1379. doi:10.1007/s00429-014-0730-z.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002A-465E-C Version Permalink: http://hdl.handle.net/21.11116/0000-0000-B69A-5
Genre: Journal Article

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 Creators:
Haberl, MG, Author
Viana da Silva, S, Author
Guest, JM, Author
Ginger, M, Author
Ghanem, A, Author
Mulle, C, Author
Oberlaender, M1, Author              
Conzelmann, K-K, Author
Frick, A, Author
Affiliations:
1Former Research Group Computational Neuroanatomy, Max Planck Institute for Biological Cybernetics, Max Planck Society, Spemannstrasse 38, 72076 Tübingen, DE, ou_2528698              

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 Abstract: Glycoprotein-deleted rabies virus (RABV ∆G) is a powerful tool for the analysis of neural circuits. Here, we demonstrate the utility of an anterograde RABV ∆G variant for novel neuroanatomical approaches involving either bulk or sparse neuronal populations. This technology exploits the unique features of RABV ∆G vectors, namely autonomous, rapid high-level expression of transgenes, and limited cytotoxicity. Our vector permits the unambiguous long-range and fine-scale tracing of the entire axonal arbor of individual neurons throughout the brain. Notably, this level of labeling can be achieved following infection with a single viral particle. The vector is effective over a range of ages (>14 months) aiding the studies of neurodegenerative disorders or aging, and infects numerous cell types in all brain regions tested. Lastly, it can also be readily combined with retrograde RABV ∆G variants. Together with other modern technologies, this tool provides new possibilities for the investigation of the anatomy and physiology of neural circuits.

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 Dates: 2015-05
 Publication Status: Published in print
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 Identifiers: DOI: 10.1007/s00429-014-0730-z
BibTex Citekey: HaberlVGGGMOCF2014
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Title: Brain Structure and Function
Source Genre: Journal
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Pages: - Volume / Issue: 220 (3) Sequence Number: - Start / End Page: 1369 - 1379 Identifier: -