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  Synthesis and Characterization of a Biotinylated Multivalent Targeted Contrast Agent

Gündüz, S., Power, A., Maier, M., Logothetis, N., & Angelovski, G. (2015). Synthesis and Characterization of a Biotinylated Multivalent Targeted Contrast Agent. ChemPlusChem, 80(3), 612-622. doi:10.1002/cplu.201402329.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002A-4758-E Version Permalink: http://hdl.handle.net/21.11116/0000-0000-FE26-8
Genre: Journal Article

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Gündüz, S1, Author              
Power, A1, 2, Author              
Maier, ME, Author
Logothetis, NK1, 2, Author              
Angelovski, G1, 3, Author              
Affiliations:
1Max Planck Institute for Biological Cybernetics, Max Planck Society, ou_1497794              
2Department Physiology of Cognitive Processes, Max Planck Institute for Biological Cybernetics, Max Planck Society, ou_1497798              
3Research Group MR Neuroimaging Agents, Max Planck Institute for Biological Cybernetics, Max Planck Society, Spemannstrasse 38, 72076 Tübingen, DE, ou_2528691              

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 Abstract: A new bimodal and multivalent dendritic contrast agent (CA) that targets the protein avidin was prepared and characterized. The tripartite lysine core was used to link the ligand biotin, the fluorescent dye, and the dendron carrying GdDOTA (DOTA=1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid) chelates for amplification of the magnetic resonance imaging (MRI) signal. The longitudinal relaxivity of this dendrimeric CA was greater than those of its GdDOTA chelate and most of the common commercial agents at the investigated high magnetic field (7 T). The capacity of the dendrimeric CA to bind to the target protein was confirmed by fluorescence measurements upon its treatment with NeutrAvidin–agarose gel or NeutrAvidin-coated microspheres and the results were compared with those of its monomeric analogue. The fluorescence intensity of monomer-treated targets was found to be greater than that from those treated with dendrimeric CA; however, a several-fold increase in the MRI signal was observed on the same samples treated with the dendrimeric CA. The inductively coupled plasma mass spectrometry analysis of the digested samples indicated somewhat higher Gd3+ content and hence slightly better binding of monomeric versus dendrimeric CA. This bimodal and multivalent targeted probe opens an avenue for the preparation of new nanosized CAs that allow high-resolution MRI of various targets, such as cellular receptors or specific cellular populations.

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 Dates: 2015-03
 Publication Status: Published in print
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 Identifiers: DOI: 10.1002/cplu.201402329
BibTex Citekey: GunduzPMLA2014
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Title: ChemPlusChem
Source Genre: Journal
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Pages: - Volume / Issue: 80 (3) Sequence Number: - Start / End Page: 612 - 622 Identifier: -