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  Comment on "Extended-resolution structured illumination imaging of endocytic and cytoskeletal dynamics"

Sahl, S. J., Balzarotti, F., Keller-Findeisen, J., Leutenegger, M., Westphal, V., Egner, A., et al. (2016). Comment on "Extended-resolution structured illumination imaging of endocytic and cytoskeletal dynamics". Science, 352(6285): 527a. doi:10.1126/science.aad7983.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002A-4FAF-0 Version Permalink: http://hdl.handle.net/21.11116/0000-0002-A70E-3
Genre: Journal Article

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2273316.pdf (Publisher version), 4MB
 
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 Creators:
Sahl, S. J.1, Author              
Balzarotti, F.1, Author              
Keller-Findeisen, J.1, Author              
Leutenegger, M.1, Author              
Westphal, V.1, Author              
Egner, A.1, Author              
Lavoie-Cardinal, F.1, Author              
Chmyrov, A.1, Author              
Grotjohann, T.1, Author              
Jakobs, S.2, Author              
Affiliations:
1Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society, ou_578627              
2Research Group of Mitochondrial Structure and Dynamics, MPI for biophysical chemistry, Max Planck Society, ou_578566              

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 Abstract: Li et al. (Research Articles, 28 August 2015, aab3500) purport to present solutions to long-standing challenges in live-cell microscopy, reporting relatively fast acquisition times in conjunction with improved image resolution. We question the methods’ reliability to visualize specimen features at sub–100-nanometer scales, because the mandatory mathematical processing of the recorded data leads to artifacts that are either difficult or impossible to disentangle from real features. We are also concerned about the chosen approach of subjectively comparing images from different super-resolution methods, as opposed to using quantitative measures.

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Language(s): eng - English
 Dates: 2016-04-292016-04-29
 Publication Status: Published in print
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 Rev. Method: Peer
 Identifiers: DOI: 10.1126/science.aad7983
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Title: Science
Source Genre: Journal
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Pages: 3 Volume / Issue: 352 (6285) Sequence Number: 527a Start / End Page: - Identifier: -