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  Growing neuronal islands on multi-electrode arrays using an accurate positioning-mu CP device.

Samhaber, R., Schottdorf, M., El Hady, A., Bröking, K., Daus, A., Thielemann, C., et al. (2016). Growing neuronal islands on multi-electrode arrays using an accurate positioning-mu CP device. Journal of Neuroscience Methods, 257, 194-203. doi:10.1016/j.jneumeth.2015.09.022.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002A-5916-D Version Permalink: http://hdl.handle.net/21.11116/0000-0000-7376-A
Genre: Journal Article

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 Creators:
Samhaber, R., Author
Schottdorf, Manuel1, Author              
El Hady, Ahmed1, Author              
Bröking, K., Author
Daus, A., Author
Thielemann, C., Author
Stühmer, W., Author
Wolf, Fred1, Author              
Affiliations:
1Research Group Theoretical Neurophysics, Max Planck Institute for Dynamics and Self-Organization, Max Planck Society, ou_2063289              

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Free keywords: mu CP; MEA; Patterning
 Abstract: Background: Multi-electrode arrays (MEAs) allow non-invasive multi-unit recording in-vitro from cultured neuronal networks. For sufficient neuronal growth and adhesion on such MEAs, substrate preparation is required. Plating of dissociated neurons on a uniformly prepared MEA's surface results in the formation of spatially extended random networks with substantial inter-sample variability. Such cultures are not optimally suited to study the relationship between defined structure and dynamics in neuronal networks. To overcome these shortcomings, neurons can be cultured with pre-defined topology by spatially structured surface modification. Spatially structuring a MEA surface accurately and reproducibly with the equipment of a typical cell-culture laboratory is challenging. New method: In this paper, we present a novel approach utilizing micro-contact printing (mu CP) combined with a custom-made device to accurately position patterns on MEAs with high precision. We call this technique AP-mu CP (accurate positioning micro-contact printing). Comparison with existing methods: Other approaches presented in the literature using mu CP for patterning either relied on facilities or techniques not readily available in a standard cell culture laboratory, or they did not specify means of precise pattern positioning. Conclusion: Here we present a relatively simple device for reproducible and precise patterning in a standard cell-culture laboratory setting. The patterned neuronal islands on MEAs provide a basis for high throughput electrophysiology to study the dynamics of single neurons and neuronal networks.

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Language(s): eng - English
 Dates: 2015-10-012016-01-15
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1016/j.jneumeth.2015.09.022
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Title: Journal of Neuroscience Methods
Source Genre: Journal
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Pages: - Volume / Issue: 257 Sequence Number: - Start / End Page: 194 - 203 Identifier: -