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  Choreography of molecular movements during ribosome progression along mRNA.

Belardinelli, R., Sharma, H., Caliskan, N., da Cunha, C. E. L., Peske, F., Wintermeyer, W., et al. (2016). Choreography of molecular movements during ribosome progression along mRNA. Nature Structural and Molecular Biology, 23(4), 342-348. doi:10.1038/nsmb.3193.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002A-54DB-A Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002C-9248-C
Genre: Journal Article

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 Creators:
Belardinelli, R.1, Author              
Sharma, H.1, Author              
Caliskan, N.1, Author              
da Cunha, C. E. L.1, Author              
Peske, F.1, Author              
Wintermeyer, W.2, Author              
Rodnina, M. V.1, Author              
Affiliations:
1Department of Physical Biochemistry, MPI for biophysical chemistry, Max Planck Society, ou_578598              
2Research Group of Ribosome Dynamics, MPI for biophysical chemistry, Max Planck Society, ou_578599              

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 Abstract: During translation elongation, ribosome translocation along an mRNA entails rotations of the ribosomal subunits, swiveling motions of the small subunit (SSU) head and stepwise movements of the tRNAs together with the mRNA. Here, we reconstructed the choreography of the collective motions of the Escherichia coli ribosome during translocation promoted by elongation factor EF-G, by recording the fluorescence signatures of nine different reporters placed on both ribosomal subunits, tRNA and mRNA. We captured an early forward swiveling of the SSU head taking place while the SSU body rotates in the opposite, clockwise direction. Backward swiveling of the SSU head starts upon tRNA translocation and continues until the post-translocation state is reached. This work places structures of translocation intermediates along a time axis and unravels principles of the motions of macromolecular machines.

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Language(s): eng - English
 Dates: 2016-03-212016-04
 Publication Status: Published in print
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 Rev. Method: Peer
 Identifiers: DOI: 10.1038/nsmb.3193
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Title: Nature Structural and Molecular Biology
Source Genre: Journal
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Pages: - Volume / Issue: 23 (4) Sequence Number: - Start / End Page: 342 - 348 Identifier: -