English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Protein cofactor competition regulates the action of a multifunctional RNA helicase in different pathways.

Heininger, A. U., Hackert, P., Andreou, A. Z., Boon, K. L., Memet, I., Prior, M., et al. (2016). Protein cofactor competition regulates the action of a multifunctional RNA helicase in different pathways. RNA Biology, 13(3), 320-330. doi:10.1080/15476286.2016.1142038.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002A-561A-A Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002C-C238-2
Genre: Journal Article

Files

show Files
hide Files
:
2281614.pdf (Publisher version), 2MB
Name:
2281614.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
:
2281614_Suppl.docx (Supplementary material), 3MB
Name:
2281614_Suppl.docx
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/vnd.openxmlformats-officedocument.wordprocessingml.document / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show

Creators

show
hide
 Creators:
Heininger, A. U., Author
Hackert, P., Author
Andreou, A. Z., Author
Boon, K. L.1, Author              
Memet, I., Author
Prior, M., Author
Clancy, A., Author
Schmidt, B., Author
Urlaub, H.2, Author              
Schleiff, E., Author
Sloan, K. E., Author
Deckers, M., Author
Lührmann, R.1, Author              
Enderlein, J., Author
Klostermeier, D., Author
Rehling, P.3, Author              
Bohnsack, M. T., Author
Affiliations:
1Department of Cellular Biochemistry, MPI for Biophysical Chemistry, Max Planck Society, ou_578576              
2Research Group of Bioanalytical Mass Spectrometry, MPI for biophysical chemistry, Max Planck Society, ou_578613              
3Max Planck Fellow Peter Rehling, ou_1298545              

Content

show
hide
Free keywords: -
 Abstract: A rapidly increasing number of RNA helicases are implicated in several distinct cellular processes, however, the modes of regulation of multifunctional RNA helicases and their recruitment to different target complexes have remained unknown. Here, we show that the distribution of the multifunctional DEAH-box RNA helicase Prp43 between its diverse cellular functions can be regulated by the interplay of its G-patch protein cofactors. We identify the orphan G-patch protein Cmg1 (YLR271W) as a novel cofactor of Prp43 and show that it stimulates the RNA binding and ATPase activity of the helicase. Interestingly, Cmg1 localizes to the cytoplasm and to the intermembrane space of mitochondria and its overexpression promotes apoptosis. Furthermore, our data reveal that different G-patch protein cofactors compete for interaction with Prp43. Changes in the expression levels of Prp43-interacting G-patch proteins modulate the cellular localization of Prp43 and G-patch protein overexpression causes accumulation of the helicase in the cytoplasm or nucleoplasm. Overexpression of several G-patch proteins also leads to defects in ribosome biogenesis that are consistent with withdrawal of the helicase from this pathway. Together, these findings suggest that the availability of cofactors and the sequestering of the helicase are means to regulate the activity of multifunctional RNA helicases and their distribution between different cellular processes.

Details

show
hide
Language(s): eng - English
 Dates: 2016-01-292016-03-03
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1080/15476286.2016.1142038
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: RNA Biology
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 13 (3) Sequence Number: - Start / End Page: 320 - 330 Identifier: -