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  A non-stem-loop CRISPR RNA is processed by dual binding Cas6.

Shao, Y. M., Richter, H., Sun, S., Sharma, K., Urlaub, H., & Randau, L. (2016). A non-stem-loop CRISPR RNA is processed by dual binding Cas6. Structure, 24(4), 547-554. doi:10.1016/j.str.2016.02.009.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002A-563D-B Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002D-1D4C-1
Genre: Journal Article

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 Creators:
Shao, Y. M., Author
Richter , H., Author
Sun, S., Author
Sharma, K.1, Author              
Urlaub, H.1, Author              
Randau, L., Author
Affiliations:
1Research Group of Bioanalytical Mass Spectrometry, MPI for biophysical chemistry, Max Planck Society, ou_578613              

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Free keywords: Protein-RNA interactions; Endoribonucleases; CRISPR RNA; Crystal structures
 Abstract: A subclass of recently discovered CRISPR repeat RNA in bacteria contains minimally recognizable structural features that facilitate an unknown mechanism of recognition and processing by the Cas6 family of endoribonucleases. Cocrystal structures of Cas6 from Methanococcus maripaludis (MmCas6b) bound with its repeat RNA revealed a dual site binding structure and a cleavage site conformation poised for phosphodiester bond breakage. Two non-interacting MmCas6b bind to two separate AAYAA motifs within the same repeat, one distal and one adjacent to the cleavage site. This bound structure potentially competes with a stable but non-productive RNA structure. At the cleavage site, MmCas6b supplies a base pair mimic to stabilize a short 2 base pair stem immediately upstream of the scissile phosphate. Complementary biochemical analyses support the dual-AAYAA binding model and a critical role of the protein-RNA base pair mimic. Our results reveal a previously unknown method of processing non-stem-loop CRISPR RNA by Cas6.

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Language(s): eng - English
 Dates: 2016-03-172016-04-05
 Publication Status: Published in print
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 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1016/j.str.2016.02.009
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Title: Structure
Source Genre: Journal
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Pages: - Volume / Issue: 24 (4) Sequence Number: - Start / End Page: 547 - 554 Identifier: -