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  Oms1 associates with cytochrome c oxidase assembly intermediates to stabilize newly synthesized Cox1.

Bareth, B., Nikolov, M., Lorenzi, I., Hildenbeutel, M., Mick, D. U., Helbig, C., et al. (2016). Oms1 associates with cytochrome c oxidase assembly intermediates to stabilize newly synthesized Cox1. Molecular Biology of the Cell, 27(10), 1570-1580. doi:10.1091/mbc.E15-12-0811.

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 Urheber:
Bareth, B., Autor
Nikolov, M.1, Autor           
Lorenzi, I., Autor
Hildenbeutel, M., Autor
Mick, D. U., Autor
Helbig, C., Autor
Urlaub, H.1, Autor           
Ott, M., Autor
Rehling, P.2, Autor           
Dennerlein, S., Autor
Affiliations:
1Research Group of Bioanalytical Mass Spectrometry, MPI for biophysical chemistry, Max Planck Society, ou_578613              
2Max Planck Fellow Peter Rehling, ou_1298545              

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 Zusammenfassung: The mitochondrial cytochromecoxidase assembles in the inner membrane from subunits of dual genetic origin. The assembly process of the enzyme is initiated by membrane insertion of the mitochondria-encoded Cox1 subunit. During complex maturation, transient assembly intermediates, consisting of structural subunits and specialized chaperone-like assembly factors, are formed. In addition, cofactors such as heme and copper have to be inserted into the nascent complex. To regulate the assembly process, the availability of Cox1 is under control of a regulatory feedback cycle, in which translation of the COX1 mRNA is stalled when assembly intermediates of Cox1 accumulate through inactivation of the translational activator Mss51. Here we have isolated a cytochromecoxidase assembly intermediate in preparatory scale fromcoa1Δmutant cells using Mss51 as a bait. We demonstrate that at this stage of assembly the complex has not yet incorporated the heme a cofactors. Using quantitative mass spectrometry, we defined the protein composition of the assembly intermediate and unexpectedly identified the putative methyltransferase Oms1 as a constituent. Our analyses show that Oms1 participates in cytochromecoxidase assembly by stabilizing newly synthesized Cox1.

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Sprache(n): eng - English
 Datum: 2016-03-302016-05-15
 Publikationsstatus: Erschienen
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 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: Expertenbegutachtung
 Identifikatoren: DOI: 10.1091/mbc.E15-12-0811
 Art des Abschluß: -

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Titel: Molecular Biology of the Cell
Genre der Quelle: Zeitschrift
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Seiten: - Band / Heft: 27 (10) Artikelnummer: - Start- / Endseite: 1570 - 1580 Identifikator: -