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  Molecular engineering of a backwards-moving myosin motor

Tsiavaliaris, G., Fujita-Becker, S., & Manstein, D. J. (2004). Molecular engineering of a backwards-moving myosin motor. Nature, 427(6974), 558-561. doi:10.1038/nature02303.

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Genre: Journal Article
Alternative Title : Molecular engineering of a backwards-moving myosin motor

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Nature_427_2004_558.pdf (Any fulltext), 219KB
 
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Tsiavaliaris, Georgios1, Author              
Fujita-Becker, Setsuko1, Author              
Manstein, Dietmar J.1, Author              
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1Emeritus Group Biophysics, Max Planck Institute for Medical Research, Max Planck Society, ou_1497712              

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 Abstract: All members of the diverse myosin superfamily have a highly conserved globular motor domain that contains the actin- and nucleotide-binding sites and produces force and movement. The light-chain-binding domain connects the motor domain to a variety of functionally specialized tail domains and amplifies small structural changes in the motor domain through rotation of a lever arm. Myosins move on polarized actin filaments either forwards to the barbed (+) or backwards to the pointed (-) end. Here, we describe the engineering of an artificial backwards-moving myosin from three pre-existing molecular building blocks. These blocks are: a forward-moving class I myosin motor domain, a directional inverter formed by a four-helix bundle segment of human guanylate-binding protein-1 and an artificial lever arm formed by two alpha-actinin repeats. Our results prove that reverse-direction movement of myosins can be achieved simply by rotating the direction of the lever arm 180 degrees.

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Language(s): eng - English
 Dates: 2003-09-032003-12-192004-02-05
 Publication Status: Published in print
 Pages: 3
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 Rev. Type: Peer
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Title: Nature
Source Genre: Journal
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Publ. Info: London : Nature Publishing Group
Pages: - Volume / Issue: 427 (6974) Sequence Number: - Start / End Page: 558 - 561 Identifier: ISSN: 0028-0836
CoNE: https://pure.mpg.de/cone/journals/resource/954925427238