ausblenden:
Schlagwörter:
MEMBRANE PROTEIN AIL; HOST-CELLS; DENDRITIC CELLS; PLAGUE VIRULENCE;
FACTOR-H; YADA; INVASIN; PSEUDOTUBERCULOSIS; INTEGRINS; BINDINGYersinia; Invasin; Type III secretion system; YadA; Integrins; Mouse
infection;
Zusammenfassung:
Injection of Yersinia outer proteins (Yops) into host cells by a type III secretion system is an important immune evasion mechanism of Yersinia enterocolitica (Ye). In this process Ye invasin (Inv) binds directly while Yersinia adhesin A (YadA) binds indirectly via extracellular matrix (ECM) proteins to beta 1 integrins on host cells. Although leukocytes turned out to be an important target of Yop injection by Ye, it was unclear which Ye adhesins and which leukocyte receptors are required for Yop injection. To explain this, we investigated the role of YadA, Inv and beta 1 integrins for Yop injection into leukocytes and their impact on the course of systemic Ye infection in mice. Ex vivo infection experiments revealed that adhesion of Ye via Inv or YadA is sufficient to promote Yop injection into leukocytes as revealed by a beta-lactamase reporter assay. Serum factors inhibit YadA-but not Inv-mediated Yop injection into B and T cells, shifting YadA-mediated Yop injection in the direction of neutrophils and other myeloid cells. Systemic Ye mouse infection experiments demonstrated that YadA is essential for Ye virulence and Yop injection into leukocytes, while Inv is dispensable for virulence and plays only a transient and minor role for Yop injection in the early phase of infection. Ye infection of mice with beta 1 integrin-depleted leukocytes demonstrated that beta 1 integrins are dispensable for YadA-mediated Yop injection into leukocytes, but contribute to Inv-mediated Yop injection. Despite reduced Yop injection into leukocytes, beta 1 integrin-deficient mice exhibited an increased susceptibility for Ye infection, suggesting an important role of beta 1 integrins in immune defense against Ye. This study demonstrates that Yop injection into leukocytes by Ye is largely mediated by YadA exploiting, as yet unknown, leukocyte receptors. (C) 2015 Published by Elsevier GmbH.
