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  Degradation Parameters from Pulse-Chase Experiments

Sin, C., Chiarugi, D., & Valleriani, A. (2016). Degradation Parameters from Pulse-Chase Experiments. PLoS One, 11(5): e0155028. doi:10.1371/journal.pone.0155028.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002A-AE04-E Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002C-7E26-3
Genre: Journal Article

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 Creators:
Sin, Celine1, Author              
Chiarugi, Davide1, Author              
Valleriani, Angelo1, Author              
Affiliations:
1Angelo Valleriani, Theorie & Bio-Systeme, Max Planck Institute of Colloids and Interfaces, Max Planck Society, ou_1863324              

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Free keywords: Open Access
 Abstract: Pulse-chase experiments are often used to study the degradation of macromolecules such as proteins or mRNA. Considerations for the choice of pulse length include the toxicity of the pulse to the cell and maximization of labeling. In the general case of non-exponential decay, varying the length of the pulse results in decay patterns that look different. Analysis of these patterns without consideration to pulse length would yield incorrect degradation parameters. Here we propose a method that constructively includes pulse length in the analysis of decay patterns and extracts the parameters of the underlying degradation process. We also show how to extract decay parameters reliably from measurements taken during the pulse phase.

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 Dates: 2016-052016
 Publication Status: Published in print
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 Identifiers: DOI: 10.1371/journal.pone.0155028
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Title: PLoS One
Source Genre: Journal
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Publ. Info: San Francisco, CA : Public Library of Science
Pages: - Volume / Issue: 11 (5) Sequence Number: e0155028 Start / End Page: - Identifier: ISSN: 1932-6203