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  Drift estimation in sparse sequential dynamic imaging, with application to nanoscale fluorescence microscopy.

Hartmann, A., Huckemann, S., Dannemann, J., Laitenberger, O., Geisler, C., Egner, A., et al. (2016). Drift estimation in sparse sequential dynamic imaging, with application to nanoscale fluorescence microscopy. Journal of the Royal Statistical Society: Series B (Statistical Methodology), 78(3), 563-587. doi:10.1111/rssb.12128.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002A-C6B4-D Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002A-C6B9-3
Genre: Journal Article

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 Creators:
Hartmann, A., Author
Huckemann, S., Author
Dannemann, J., Author
Laitenberger, O., Author
Geisler, C., Author
Egner, A., Author
Munk, A.1, Author              
Affiliations:
1Research Group of Statistical Inverse-Problems in Biophysics, MPI for biophysical chemistry, Max Planck Society, ou_1113580              

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Free keywords: Asymptotic normality; Drift estimation; Image registration; M-estimation; Nanoscale fluorescence microscopy; Registration; Semiparametrics; Sparsity; Superresolution microscopy
 Abstract: A major challenge in many modern superresolution fluorescence microscopy techniques at the nanoscale lies in the correct alignment of long sequences of sparse but spatially and temporally highly resolved images. This is caused by the temporal drift of the protein structure, e.g. due to temporal thermal inhomogeneity of the object of interest or its supporting area during the observation process. We develop a simple semiparametric model for drift correction in single-marker switching microscopy. Then we propose an M-estimator for the drift and show its asymptotic normality. This is used to correct the final image and it is shown that this purely statistical method is competitive with state of the art calibration techniques which require the incorporation of fiducial markers in the specimen. Moreover, a simple bootstrap algorithm allows us to quantify the precision of the drift estimate and its effect on the final image estimation. We argue that purely statistical drift correction is even more robust than fiducial tracking, rendering the latter superfluous in many applications. The practicability of our method is demonstrated by a simulation study and by a single-marker switching application. This serves as a prototype for many other typical imaging techniques where sparse observations with high temporal resolution are blurred by motion of the object to be reconstructed.

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Language(s): eng - English
 Dates: 2015-07-062016-06
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1111/rssb.12128
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Title: Journal of the Royal Statistical Society: Series B (Statistical Methodology)
Source Genre: Journal
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Pages: - Volume / Issue: 78 (3) Sequence Number: - Start / End Page: 563 - 587 Identifier: -