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  Loading capacity versus enzyme activity in anisotropic and spherical calcium carbonate microparticles.

Donatan, S., Yashchenok, A., Khan, N., Parakhonskiy, B., Cocquyt, M., Pinchasik, B. E., et al. (2016). Loading capacity versus enzyme activity in anisotropic and spherical calcium carbonate microparticles. ACS Applied Materials and Interfaces, 8(22), 14284-14292. doi:10.1021/acsami.6b03492.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002A-CB3A-E Version Permalink: http://hdl.handle.net/21.11116/0000-0000-76B2-2
Genre: Journal Article

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2304277.pdf (Publisher version), 6MB
 
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 Creators:
Donatan, S., Author
Yashchenok, A., Author
Khan, N.1, Author              
Parakhonskiy, B., Author
Cocquyt, M., Author
Pinchasik, B. E., Author
Khalenkow, D., Author
Möhwald, H., Author
Konrad, M.1, Author              
Affiliations:
1Research Group of Enzyme Biochemistry, MPI for biophysical chemistry, Max Planck Society, ou_578612              

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Free keywords: calcium carbonate; enzyme; enzyme-catalyzed reaction; polyelectrolyte; vaterite
 Abstract: A new method of fabrication of calcium carbonate microparticles of ellipsoidal, rhomboidal, and spherical geometries is reported by adjusting the relative concentration ratios of the initial salt solutions and/or the ethylene glycol content in the reaction medium. Morphology, porosity, crystallinity, and loading capacity of synthesized CaCO3 templates were characterized in detail. Particles harboring dextran or the enzyme guanylate kinase were obtained through encapsulation of these macromolecules using the layer-by-layer assembly technique to deposit positively and negatively charged polymers on these differently shaped CaCO3 templates and were characterized by confocal laser scanning fluorescence microscopy, fluorometric techniques, and enzyme activity measurements. The enzymatic activity, an important application of such porous particles and containers, has been analyzed in comparison with the loading capacity and geometry. Our results reveal that the particles' shape influences morphology of particles and that, as a result, affects the activity of the encapsulated enzymes, in addition to the earlier reported influence on cellular uptake. These particles are promising candidates for efficient drug delivery due to their relatively high loading capacity, biocompatibility, and easy fabrication and handling.

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Language(s): eng - English
 Dates: 2016-05-112016-06-08
 Publication Status: Published in print
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 Rev. Method: Peer
 Identifiers: DOI: 10.1021/acsami.6b03492
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Title: ACS Applied Materials and Interfaces
Source Genre: Journal
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Pages: - Volume / Issue: 8 (22) Sequence Number: - Start / End Page: 14284 - 14292 Identifier: -