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  The target of the DEAH-box NTP triphosphatase Prp43 in Saccharomyces cerevisiae spliceosomes is the U2 snRNP-intron interaction.

Fourmann, J. B., Dybkov, O., Agafonov, D. E., Tauchert, M. J., Urlaub, H., Ficner, R., et al. (2016). The target of the DEAH-box NTP triphosphatase Prp43 in Saccharomyces cerevisiae spliceosomes is the U2 snRNP-intron interaction. eLife, 5: e15564. doi:10.7554/eLife.15564.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002A-DB03-F Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002A-DB07-7
Genre: Journal Article

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 Creators:
Fourmann, J. B.1, Author              
Dybkov, O.1, Author              
Agafonov, D. E.1, Author              
Tauchert, M. J., Author
Urlaub, H.2, Author              
Ficner, R., Author
Fabrizio, P.1, Author              
Lührmann, R.1, Author              
Affiliations:
1Department of Cellular Biochemistry, MPI for biophysical chemistry, Max Planck Society, ou_578576              
2Research Group of Bioanalytical Mass Spectrometry, MPI for biophysical chemistry, Max Planck Society, ou_578613              

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Free keywords: Human; S. cerevisiae; biochemistry; chromosomes; genes; helicases; spliceosome
 Abstract: The DEAH-box NTPase Prp43 and its cofactors Ntr1 and Ntr2 form the NTR complex and are required for disassembling intron-lariat spliceosomes (ILS) and defective earlier spliceosomes. However, the Prp43 binding site in the spliceosome and its target(s) are unknown. We show that Prp43 fused to Ntr1's G-patch motif (Prp43_Ntr1GP) is as efficient as the NTR in ILS disassembly, yielding identical dissociation products and recognizing its natural ILS target even in the absence of Ntr1's C-terminal-domain (CTD) and Ntr2. Unlike the NTR, Prp43_Ntr1GP disassembles earlier spliceosomal complexes (A, B, B(act)), indicating that Ntr2/Ntr1-CTD prevents NTR from disrupting properly assembled spliceosomes other than the ILS. The U2 snRNP-intron interaction is disrupted in all complexes by Prp43_Ntr1GP, and in the spliceosome contacts U2 proteins and the pre-mRNA, indicating that the U2 snRNP-intron interaction is Prp43's major target.

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Language(s): eng - English
 Dates: 2016-04-26
 Publication Status: Published online
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 Rev. Method: Peer
 Identifiers: DOI: 10.7554/eLife.15564
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Title: eLife
Source Genre: Journal
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Pages: 17 Volume / Issue: 5 Sequence Number: e15564 Start / End Page: - Identifier: -