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  DNA Nanostructures on Membranes as Tools for Synthetic Biology

Czogalla, A., Franquelim, H. G., & Schwille, P. (2016). DNA Nanostructures on Membranes as Tools for Synthetic Biology. Biophysical Journal, 110(8), 1698-1707. doi:10.1016/j.bpj.2016.03.015.

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 Creators:
Czogalla, Aleksander1, Author
Franquelim, Henri G.2, Author              
Schwille, Petra2, Author              
Affiliations:
1external, ou_persistent22              
2Schwille, Petra / Cellular and Molecular Biophysics, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565169              

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Free keywords: SUPPORTED LIPID-BILAYERS; LIPOPHILIC NUCLEIC-ACIDS; VESICLE FUSION; ANCHORED DNA; PHOSPHOLIPID-MEMBRANES; ORIGAMI NANOSTRUCTURES; NANOSCALE SHAPES; MODEL SYSTEMS; FOLDING DNA; OLIGONUCLEOTIDES
 Abstract: Over the last decade, functionally designed DNA nanostructures applied to lipid membranes prompted important achievements in the fields of biophysics and synthetic biology. Taking advantage of the universal rules for self-assembly of complementary oligonucleotides, DNA has proven to be an extremely versatile biocompatible building material on the nanoscale. The possibility to chemically integrate functional groups into oligonucleotides, most notably with lipophilic anchors, enabled a widespread usage of DNA as a viable alternative to proteins with respect to functional activity on membranes. As described throughout this review, hybrid DNA-lipid nanostructures can mediate events such as vesicle docking and fusion, or selective partitioning of molecules into phase-separated membranes. Moreover, the major benefit of DNA structural constructs, such as DNA tiles and DNA origami, is the reproducibility and simplicity of their design. DNA nanotechnology can produce functional structures with subnanometer precision and allow for a tight control over their biochemical functionality, e.g., interaction partners. DNA-based membrane nanopores and origami structures able to assemble into two-dimensional networks on top of lipid bilayers are recent examples of the manifold of complex devices that can be achieved. In this review, we will shortly present some of the potentially most relevant avenues and accomplishments of membrane-anchored DNA nanostructures for investigating, engineering, and mimicking lipid membrane-related biophysical processes.

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Language(s): eng - English
 Dates: 2016
 Publication Status: Published in print
 Pages: 10
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: ISI: 000374859600004
DOI: 10.1016/j.bpj.2016.03.015
 Degree: -

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Title: Biophysical Journal
  Other : Biophys. J.
Source Genre: Journal
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Publ. Info: Cambridge, Mass. : Cell Press
Pages: - Volume / Issue: 110 (8) Sequence Number: - Start / End Page: 1698 - 1707 Identifier: ISSN: 0006-3495
CoNE: https://pure.mpg.de/cone/journals/resource/954925385117