非表示:
キーワード:
HISTONE METHYLTRANSFERASE ACTIVITY; METHYL-LYSINE-BINDING; GROUP PROTEIN
COMPLEX; SEX-COMB; DNA-BINDING; REPRESSIVE COMPLEX; TRANSCRIPTIONAL
REPRESSION; CHROMATIN-STRUCTURE; STRUCTURAL BASIS; GENE-EXPRESSIONPRC1; PhoRC; SAM domain; Polycomb response element; Drosophila;
要旨:
Polycomb group (PcG) protein complexes repress transcription by modifying target gene chromatin. In Drosophila, this repression requires association of PcG protein complexes with cis-regulatory Polycomb response elements (PREs), but the interactions permitting formation of these assemblies are poorly understood. We show that the Sfmbt subunit of the DNA-binding Pho-repressive complex (PhoRC) and the Scm subunit of the canonical Polycomb-repressive complex 1 (PRC1) directly bind each other through their SAM domains. The 1.9 angstrom crystal structure of the Scm-SAM: Sfmbt-SAM complex reveals the recognition mechanism and shows that Sfmbt-SAM lacks the polymerization capacity of the SAM domains of Scm and its PRC1 partner subunit, Ph. Functional analyses in Drosophila demonstrate that Sfmbt-SAM and Scm-SAM are essential for repression and that PhoRC DNA binding is critical to initiate PRC1 association with PREs. Together, this suggests that PRE-tethered Sfmbt-SAM nucleates PRC1 recruitment and that Scm-SAM/Ph-SAM-mediated polymerization then results in the formation of PRC1-compacted chromatin.
