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  Quantitative super-resolution imaging with qPAINT

Jungmann, R., Avendano, M. S., Dai, M., Woehrstein, J. B., Agasti, S. S., Feiger, Z., et al. (2016). Quantitative super-resolution imaging with qPAINT. NATURE METHODS, 13(5), 439-442. doi:10.1038/NMETH.3804.

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 Creators:
Jungmann, Ralf1, Author              
Avendano, Maier S.2, Author
Dai, Mingjie2, Author
Woehrstein, Johannes B.1, Author              
Agasti, Sarit S.2, Author
Feiger, Zachary2, Author
Rodal, Avital2, Author
Yin, Peng2, Author
Affiliations:
1Jungmann, Ralf / Molecular Imaging and Bionanotechnology, Max Planck Institute of Biochemistry, Max Planck Society, ou_2149679              
2external, ou_persistent22              

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Free keywords: NUCLEAR-PORE COMPLEX; SPATIAL-ORGANIZATION; RNA-POLYMERASE; DNA-PAINT; MICROSCOPY; MOLECULES; PROBES; FLUORESCENCE; BRUCHPILOT; MATURATION
 Abstract: Counting molecules in complexes is challenging, even with super-resolution microscopy. Here, we use the programmable and specific binding of dye-labeled DNA probes to count integer numbers of targets. This method, called quantitative points accumulation in nanoscale topography (qPAINT), works independently of dye photophysics for robust counting with high precision and accuracy over a wide dynamic range. qPAINT was benchmarked on DNA nanostructures and demonstrated for cellular applications by quantifying proteins in situ and the number of single-molecule FISH probes bound to an mRNA target.

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Language(s): eng - English
 Dates: 2016
 Publication Status: Published in print
 Pages: 8
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: ISI: 000374981900017
DOI: 10.1038/NMETH.3804
 Degree: -

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Title: NATURE METHODS
Source Genre: Journal
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Publ. Info: MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND : NATURE PUBLISHING GROUP
Pages: - Volume / Issue: 13 (5) Sequence Number: - Start / End Page: 439 - 442 Identifier: ISSN: 1548-7091