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  Mechanism of Regulation of Intrachromatid Recombination and Long-Range Chromosome Interactions in Saccharomyces cerevisiae

Zaman, S., Choudhury, M., Jiang, J. C., Srivastava, P., Mohanty, B. K., Danielson, C., et al. (2016). Mechanism of Regulation of Intrachromatid Recombination and Long-Range Chromosome Interactions in Saccharomyces cerevisiae. Molecular and Cellular Biology (Washington, DC), 36(10), 1451-1463. doi:10.1128/MCB.01100-15.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002A-E383-C Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002B-9E4E-B
Genre: Journal Article

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 Creators:
Zaman, Shamsu1, Author
Choudhury, Malay1, Author
Jiang, James C.1, Author
Srivastava, Pankaj1, Author
Mohanty, Bidyut K.1, Author
Danielson, Christopher1, Author
Humphrey, Sean2, Author              
Jazwinski, S. Michal1, Author
Bastia, Deepak1, Author
Affiliations:
1external, ou_persistent22              
2Mann, Matthias / Proteomics and Signal Transduction, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565159              

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Free keywords: YEAST RIBOSOMAL DNA; SILENCING PROTEIN SIR2; RNA-POLYMERASE-I; REPLICATION FORK; S-CEREVISIAE; RDNA; FOB1; COMPLEX; ARREST; GENES
 Abstract: The NAD-dependent histone deacetylase Sir2 controls ribosomal DNA (rDNA) silencing by inhibiting recombination and RNA polymerase II-catalyzed transcription in the rDNA of Saccharomyces cerevisiae. Sir2 is recruited to nontranscribed spacer 1 (NTS1) of the rDNA array by interaction between the RENT (regulation of nucleolar silencing and telophase exit) complex and the replication terminator protein Fob1. The latter binds to its cognate sites, called replication termini (Ter) or replication fork barriers (RFB), that are located in each copy of NTS1. This work provides new mechanistic insights into the regulation of rDNA silencing and intrachromatid recombination by showing that Sir2 recruitment is stringently regulated by Fob1 phosphorylation at specific sites in its C-terminal domain (C-Fob1), which also regulates long-range Ter-Ter interactions. We show further that long-range Fob1-mediated Ter-Ter interactions in trans are downregulated by Sir2. These regulatory mechanisms control intrachromatid recombination and the replicative life span (RLS).

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Language(s): eng - English
 Dates: 2016
 Publication Status: Published in print
 Pages: 13
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: ISI: 000375233000003
DOI: 10.1128/MCB.01100-15
 Degree: -

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Title: Molecular and Cellular Biology (Washington, DC)
  Other : Mol Cell Biol
Source Genre: Journal
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Publ. Info: American Society for Microbiology (ASM)
Pages: - Volume / Issue: 36 (10) Sequence Number: - Start / End Page: 1451 - 1463 Identifier: ISSN: 0270-7306
CoNE: /journals/resource/954925502188