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  The native microbiome of the nematode Caenorhabditis elegans: gateway to a new host-microbiome model

Dirksen, P., Marsh, S. A., Braker, I., Heitland, N., Wagner, S., Nakad, R., et al. (2016). The native microbiome of the nematode Caenorhabditis elegans: gateway to a new host-microbiome model. BMC Biology, 14: 38. doi:10.1186/s12915-016-0258-1.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002A-E42E-8 Version Permalink: http://hdl.handle.net/21.11116/0000-0005-2042-C
Genre: Journal Article

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 Creators:
Dirksen, Philipp, Author
Marsh, Sarah Arnaud, Author
Braker, Ines, Author
Heitland, Nele, Author
Wagner, Sophia, Author
Nakad, Rania, Author
Mader, Sebastian, Author
Petersen, Carola, Author
Kowallik, Vienna1, Author              
Rosenstiel, Philip, Author
Felix, Marie-Anne, Author
Schulenburg, Hinrich2, Author              
Affiliations:
1Emmy-Noether-Group Community Dynamics, Department Evolutionary Ecology, Max Planck Institute for Evolutionary Biology, Max Planck Society, ou_2068285              
2External Organizations, ou_persistent22              

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Free keywords: Caenorhabditis elegans; Metaorganism; Microbiome; Pseudomonas; Ochrobactrum; Stenotrophomonas; Sphingomonas; Holobiont; Antifungal defense
 Abstract: BACKGROUND: Host-microbe associations underlie many key processes of host development, immunity, and life history. Yet, none of the current research on the central model species Caenorhabditis elegans considers the worm's natural microbiome. Instead, almost all laboratories exclusively use the canonical strain N2 and derived mutants, maintained through routine bleach sterilization in monoxenic cultures with an E. coli strain as food. Here, we characterize for the first time the native microbiome of C. elegans and assess its influence on nematode life history characteristics. RESULTS: Nematodes sampled directly from their native habitats carry a species-rich bacterial community, dominated by Proteobacteria such as Enterobacteriaceae and members of the genera Pseudomonas, Stenotrophomonas, Ochrobactrum, and Sphingomonas. The C. elegans microbiome is distinct from that of the worm's natural environment and the congeneric species C. remanei. Exposure to a derived experimental microbiome revealed that bacterial composition is influenced by host developmental stage and genotype. These experiments also showed that the microbes enhance host fitness under standard and also stressful conditions (e.g., high temperature and either low or high osmolarity). Taking advantage of the nematode's transparency, we further demonstrate that several Proteobacteria are able to enter the C. elegans gut and that an Ochrobactrum isolate even seems to be able to persist in the intestines under stressful conditions. Moreover, three Pseudomonas isolates produce an anti-fungal effect in vitro which we show can contribute to the worm's defense against fungal pathogens in vivo. CONCLUSION: This first systematic analysis of the nematode's native microbiome reveals a species-rich bacterial community to be associated with C. elegans, which is likely of central importance for our understanding of the worm's biology. The information acquired and the microbial isolates now available for experimental work establishes C. elegans as a tractable model for the in-depth dissection of host-microbiome interactions.

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Language(s): eng - English
 Dates: 2015-12-112016-04-192016-05-092016-05
 Publication Status: Published in print
 Pages: -
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 Table of Contents: -
 Rev. Method: -
 Identifiers: DOI: 10.1186/s12915-016-0258-1
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Title: BMC Biology
Source Genre: Journal
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Publ. Info: BioMed Central
Pages: - Volume / Issue: 14 Sequence Number: 38 Start / End Page: - Identifier: ISSN: 1741-7007
CoNE: https://pure.mpg.de/cone/journals/resource/111071069889000