English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Architecture and RNA binding of the human negative elongation factor.

Vos, S. M., Pöllmann, D., Caizzi, L., Hofmann, K. B., Rombaut, P., Zimniak, T., et al. (2016). Architecture and RNA binding of the human negative elongation factor. eLife, 5: e14981. doi:10.7554/eLife.14981.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002A-E444-1 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002D-2967-8
Genre: Journal Article

Files

show Files
hide Files
:
2305918.pdf (Publisher version), 7MB
Name:
2305918.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
:
2305918-Suppl.pdf (Supplementary material), 11MB
Name:
2305918-Suppl.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show
hide
Description:
-

Creators

show
hide
 Creators:
Vos, S. M.1, Author              
Pöllmann, D.1, Author              
Caizzi, L.1, Author              
Hofmann, K. B.1, Author              
Rombaut, P., Author
Zimniak, T., Author
Herzog, F., Author
Cramer, P.1, Author              
Affiliations:
1Department of Molecular Biology, MPI for Biophysical Chemistry, Max Planck Society, ou_1863498              

Content

show
hide
Free keywords: -
 Abstract: Transcription regulation in metazoans often involves promoter-proximal pausing of RNA polymerase (Pol) II, which requires the 4-subunit negative elongation factor (NELF). Here we discern the functional architecture of human NELF through X-ray crystallography, protein crosslinking, biochemical assays, and RNA crosslinking in cells. We identify a NELF core subcomplex formed by conserved regions in subunits NELF-A and NELF-C, and resolve its crystal structure. The NELF-AC subcomplex binds single-stranded nucleic acids in vitro, and NELF-C associates with RNA in vivo. A positively charged face of NELF-AC is involved in RNA binding, whereas the opposite face of the NELF-AC subcomplex binds NELF-B. NELF-B is predicted to form a HEAT repeat fold, also binds RNA in vivo, and anchors the subunit NELF-E, which is confirmed to bind RNA in vivo. These results reveal the three-dimensional architecture and three RNA-binding faces of NELF.

Details

show
hide
Language(s): eng - English
 Dates: 2016-06-10
 Publication Status: Published online
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.7554/eLife.14981
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: eLife
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: 27 Volume / Issue: 5 Sequence Number: e14981 Start / End Page: - Identifier: -