English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Two-photon excited autofluorescence imaging of human retinal pigment epithelial cells

Han, M., Bindewald-Wittich, A., Holz, F. G., Giese, G., Niemz, M. H., Snyder, S. R., et al. (2006). Two-photon excited autofluorescence imaging of human retinal pigment epithelial cells. Journal of Biomedical Optics, 11(1), 10501-10501. doi:10.1117/1.2171649.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002A-E8EE-9 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002A-E8FB-B
Genre: Journal Article
Alternative Title : Two-photon excited autofluorescence imaging of human retinal pigment epithelial cells

Files

show Files
hide Files
:
JBiomedicalOpt_11_2006_10501.pdf (Any fulltext), 183KB
 
File Permalink:
-
Name:
JBiomedicalOpt_11_2006_10501.pdf
Description:
-
Visibility:
Restricted (Max Planck Institute for Medical Research, MHMF; )
MIME-Type / Checksum:
application/pdf
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show

Creators

show
hide
 Creators:
Han, Meng, Author
Bindewald-Wittich, Almut, Author
Holz, Frank G., Author
Giese, Günter1, Author              
Niemz, Markolf H., Author
Snyder, Sarah Rebecca1, Author              
Sun, Hui, Author
Yu, Jiayi, Author
Agopov, Mikael1, Author              
La Schiazza, Olivier1, Author              
Bille, Josef F., Author
Affiliations:
1Department of Biomedical Optics, Max Planck Institute for Medical Research, Max Planck Society, ou_1497699              

Content

show
hide
Free keywords: multiphoton; microscopy; retina; autofluorescence
 Abstract: Degeneration of retinal pigment epithelial (RPE) cells severely impairs the visual function of retina photoreceptors. However, little is known about the events that trigger the death of RPE cells at the subcellular level. Two-photon excited autofluorescence (TPEF) imaging of RPE cells proves to be well suited to investigate both the morphological and the spectral characteristics of the human RPE cells. The dominant fluorophores of autofluorescence derive from lipofuscin (LF) granules that accumulate in the cytoplasm of the RPE cells with increasing age. Spectral TPEF imaging reveals the existence of abnormal LF granules with blue shifted autofluorescence in RPE cells of aging patients and brings new insights into the complicated composition of the LF granules. Based on a proposed two-photon laser scanning ophthalmoscope, TPEF imaging of the living retina may be valuable for diagnostic and pathological studies of age related eye diseases.

Details

show
hide
Language(s): eng - English
 Dates: 2005-12-092005-11-042005-12-282006-02-102006-02-10
 Publication Status: Published in print
 Pages: 3
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Journal of Biomedical Optics
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: Bellingham, WA : Published by SPIE--the International Society for Optical Engineering in cooperation with International Biomedical Optics Society
Pages: - Volume / Issue: 11 (1) Sequence Number: - Start / End Page: 10501 - 10501 Identifier: ISSN: 1083-3668
CoNE: https://pure.mpg.de/cone/journals/resource/954925607859