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  Viral vectors: a wide range of choices and high levels of service

Osten, P., Grinevich, V., & Cetin, A. (2007). Viral vectors: a wide range of choices and high levels of service. In R. Feil (Ed.), Conditional mutagenesis: an approach to disease models (pp. 177-202). Berlin: Springer.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002A-EC47-0 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002A-EC48-E
Genre: Book Chapter
Alternative Title : Viral vectors: a wide range of choices and high levels of service

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HdbkExperimentPharmacol_178_2007_177.pdf (Any fulltext), 322KB
 
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 Creators:
Osten, Pavel1, Author              
Grinevich, Valery1, Author              
Cetin, Ali1, Author              
Affiliations:
1Department of Molecular Neurobiology, Max Planck Institute for Medical Research, Max Planck Society, ou_1497704              

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Free keywords: Recombinant viral vectors; Gene delivery; Conditional gene expression; Conditional gene knock-down
 Abstract: Viruses are intracellular parasites with simple DNA or RNA genomes. Virus life revolves around three steps: infection of a host cell, replication of its genome within the host cell environment, and formation of new virions; this process is often but not always associated with pathogenic effects against the host organism. Since the mid-1980s, the main goal of viral vectorology has been to develop recombinant viral vectors for long-term gene delivery to mammalian cells, with minimal associated toxicity. Today, several viral vector systems are close to achieving this aim, providing stable transgenic expression in many different cell types and tissues. Here we review application characteristics of four vector systems, derived from adeno-associated viruses, adenoviruses, retroviruses and herpes simplex virus-1, for in vivo gene delivery. We discuss the transfer capacity of the expression vectors, the stability of their transgenic expression, the tropism of the recombinant viruses, the likelihood of induction of immunotoxicity, and the ease (or difficulty) of the virus production. In the end, we discuss applications of these vectors for delivery of three molecular systems for conditional mutagenesis, two for inducible transcriptional control of transgenic expression (the tet and the dimerizer systems), and the third one for inducible control of endogenous gene expression based on RNA interference.

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Language(s): eng - English
 Dates: 2007
 Publication Status: Published in print
 Pages: 26
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Degree: -

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Title: Conditional mutagenesis : an approach to disease models
Source Genre: Book
 Creator(s):
Abuin, F., Author
Feil, R., Editor
Affiliations:
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Publ. Info: Berlin : Springer
Pages: - Volume / Issue: - Sequence Number: - Start / End Page: 177 - 202 Identifier: ISBN: 978-3-540-35109-2