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  Reconstruction of firing rate changes across neuronal populations by temporally deconvolved Ca2+ imaging

Yaksi, E., & Friedrich, R. W. (2006). Reconstruction of firing rate changes across neuronal populations by temporally deconvolved Ca2+ imaging. Nature methods, 3(5), 377-383. doi:10.1038/nmeth874.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002A-EE57-7 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002A-EE58-5
Genre: Journal Article
Alternative Title : Reconstruction of firing rate changes across neuronal populations by temporally deconvolved Ca2+ imaging

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 Creators:
Yaksi, Emre1, Author              
Friedrich, Rainer W.1, Author              
Affiliations:
1Department of Biomedical Optics, Max Planck Institute for Medical Research, Max Planck Society, ou_1497699              

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 Abstract: Methods to record action potential (AP) firing in many individual neurons are essential to unravel the function of complex neuronal circuits in the brain. A promising approach is bolus loading of Ca(2+) indicators combined with multiphoton microscopy. Currently, however, this technique lacks cell-type specificity, has low temporal resolution and cannot resolve complex temporal firing patterns. Here we present simple solutions to these problems. We identified neuron types by colocalizing Ca(2+) signals of a red-fluorescing indicator with genetically encoded markers. We reconstructed firing rate changes from Ca(2+) signals by temporal deconvolution. This technique is efficient, dramatically enhances temporal resolution, facilitates data interpretation and permits analysis of odor-response patterns across thousands of neurons in the zebrafish olfactory bulb. Hence, temporally deconvolved Ca(2+) imaging (TDCa imaging) resolves limitations of current optical recording techniques and is likely to be widely applicable because of its simplicity, robustness and generic principle.

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Language(s): eng - English
 Dates: 2005-12-122006-03-202006-04-202006-05-01
 Publication Status: Published in print
 Pages: 7
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Degree: -

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Title: Nature methods
  Other : Nature methods
Source Genre: Journal
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Publ. Info: New York, NY : Nature Pub. Group
Pages: - Volume / Issue: 3 (5) Sequence Number: - Start / End Page: 377 - 383 Identifier: ISSN: 1548-7091
CoNE: https://pure.mpg.de/cone/journals/resource/111088195279556