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  Identifications of novel mechanisms in breast cancer cells involving duct-like multicellular spheroid formation after exposure to the Random Positioning Machine

Kopp, S., Slumstrup, L., Corydon, T. J., Sahana, J., Aleshcheva, G., Islam, T., et al. (2016). Identifications of novel mechanisms in breast cancer cells involving duct-like multicellular spheroid formation after exposure to the Random Positioning Machine. SCIENTIFIC REPORTS, 6: 26887. doi:10.1038/srep26887.

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 Creators:
Kopp, Sascha1, Author
Slumstrup, Lasse1, Author
Corydon, Thomas J.1, Author
Sahana, Jayashree1, Author
Aleshcheva, Ganna1, Author
Islam, Tawhidul1, Author
Magnusson, Nils E.1, Author
Wehland, Markus1, Author
Bauer, Johann2, Author              
Infanger, Manfred1, Author
Grimm, Daniela1, Author
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1external, ou_persistent22              
2Scientific Service Groups, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565170              

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Free keywords: ENDOTHELIAL GROWTH-FACTOR; THYROID-CARCINOMA CELLS; SIMULATED MICROGRAVITY; EXTRACELLULAR-MATRIX; GENE-EXPRESSION; 3-DIMENSIONAL GROWTH; INCREASES APOPTOSIS; LYMPHOCYTES JURKAT; MAMMALIAN-CELLS; LINE MCF-7
 Abstract: Many cell types form three-dimensional aggregates (MCS; multicellular spheroids), when they are cultured under microgravity. MCS often resemble the organ, from which the cells have been derived. In this study we investigated human MCF-7 breast cancer cells after a 2 h-, 4 h-, 16 h-, 24 h-and 5d-exposure to a Random Positioning Machine (RPM) simulating microgravity. At 24 h few small compact MCS were detectable, whereas after 5d many MCS were floating in the supernatant above the cells, remaining adherently (AD). The MCS resembled the ducts formed in vivo by human epithelial breast cells. In order to clarify the underlying mechanisms, we harvested MCS and AD cells separately from each RPM-culture and measured the expression of 29 selected genes with a known involvement in MCS formation. qPCR analyses indicated that cytoskeletal genes were unaltered in short-term samples. IL8, VEGFA, and FLT1 were upregulated in 2 h/4 h AD-cultures. The ACTB, TUBB, EZR, RDX, FN1, VEGFA, FLK1 Casp9, Casp3, PRKCA mRNAs were downregulated in 5d-MCS-samples. ESR1 was upregulated in AD, and PGR1 in both phenotypes after 5d. A pathway analysis revealed that the corresponding gene products are involved in organization and regulation of the cell shape, in cell tip formation and membrane to membrane docking.

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Language(s): eng - English
 Dates: 2016
 Publication Status: Published online
 Pages: 18
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: ISI: 000376749700001
DOI: 10.1038/srep26887
 Degree: -

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Title: SCIENTIFIC REPORTS
Source Genre: Journal
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Publ. Info: MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND : NATURE PUBLISHING GROUP
Pages: - Volume / Issue: 6 Sequence Number: 26887 Start / End Page: - Identifier: ISSN: 2045-2322