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  Loss of AP-2delta reduces retinal ganglion cell numbers and axonal projections to the superior colliculus

Li, X., Gaillard, F., Monckton, E. A., Glubrecht, D. D., Persad, A. R. L., Moser, M., et al. (2016). Loss of AP-2delta reduces retinal ganglion cell numbers and axonal projections to the superior colliculus. Molecular Brain, 9: 62. doi:10.1186/s13041-016-0244-0.

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 Creators:
Li, Xiaodong1, Author
Gaillard, Frederic1, Author
Monckton, Elizabeth A.1, Author
Glubrecht, Darryl D.1, Author
Persad, Amit R. L.1, Author
Moser, Markus2, Author              
Sauve, Yves1, Author
Godbout, Roseline1, Author
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1external, ou_persistent22              
2Fässler, Reinhard / Molecular Medicine, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565147              

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Free keywords: TRANSCRIPTION FACTOR AP-2; DEVELOPING CHICK RETINA; INFERIOR COLLICULUS; NEURAL CREST; RETINOCOLLICULAR PROJECTIONS; SOMATOSENSORY NEURONS; POSTNATAL-DEVELOPMENT; PROMOTER ACTIVITY; FACTOR AP-2-BETA; TECTAL LESIONSAP-2; Transcription factor; Retina; Ganglion cells; Axon; Brain; Superior colliculus; Electrophysiology;
 Abstract: Background: AP-2 delta is the most divergent member of the Activating Protein-2 (TFAP2) family of transcription factors. AP-2 delta is restricted to specific regions of the CNS, including a subset of ganglion cells in the retina. Retinal ganglion cells (RGCs), the only output neurons of the retina, are responsible for transmitting the visual signal to the brain. Results: AP-2 delta knockout results in loss of Brn3c (Pou4f3) expression in AP-2 delta -positive RGCs. While AP-2 delta-/- mice have morphologically normal retinas at birth, there is a significant reduction in retinal ganglion cell numbers by P21, after eye opening. Chromatin immunoprecipitation indicates that Brn3c is a target of AP-2 delta in the retina. Using fluorochrome-conjugated cholera toxin subunit B to trace ganglion cell axons from the eye to the major visual pathways in the brain, we found 87 % and 32 % decreases in ipsilateral and contralateral projections, respectively, to the superior colliculus in AP-2 delta-/- mice. In agreement with anatomical data, visually evoked responses recorded from the brain confirmed that retinal outputs to the brain are compromised. Conclusions: AP-2 delta is important for the maintenance of ganglion cell numbers in the retina. Loss of AP-2 delta alters retinal axonal projections to visual centers of the brain, with ipsilaterial projections to the superior colliculus being the most dramatically affected. Our results have important implications for integration of the visual signal at the superior colliculus.

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Language(s): eng - English
 Dates: 2016
 Publication Status: Published online
 Pages: 21
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: ISI: 000377189500001
DOI: 10.1186/s13041-016-0244-0
 Degree: -

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Title: Molecular Brain
Source Genre: Journal
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Publ. Info: London : BioMed Central
Pages: - Volume / Issue: 9 Sequence Number: 62 Start / End Page: - Identifier: Other: 1756-6606
CoNE: https://pure.mpg.de/cone/journals/resource/1756-6606