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  Contribution of iron and protein contents from rat brain subcellular fractions to MR phase imaging

Leutritz, T., Hilfert, L., Busse, U., Smalla, K.-H., Speck, O., & Zhong, K. (2017). Contribution of iron and protein contents from rat brain subcellular fractions to MR phase imaging. Magnetic Resonance in Medicine, 77(5), 2028-2039. doi:10.1002/mrm.26288.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002B-02D0-A Version Permalink: http://hdl.handle.net/21.11116/0000-0003-BB69-5
Genre: Journal Article

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 Creators:
Leutritz, Tobias1, Author              
Hilfert, L.2, Author
Busse, U.3, Author
Smalla, K.-H.4, 5, Author
Speck, O.1, 4, 5, 6, Author
Zhong, K.1, 7, Author
Affiliations:
1Department of Biomedical Magnetic Resonance, Institute of Experimental Physics, Otto von Guericke University Magdeburg, Germany, ou_persistent22              
2Institute for Chemistry, Otto von Guericke University Magdeburg, Germany, ou_persistent22              
3Institute of Instrumental and Environmental Technology, Otto von Guericke University Magdeburg, Germany, ou_persistent22              
4Center for Behavioral Brain Sciences, Magdeburg, Germany, ou_persistent22              
5Leibniz Institute for Neurobiology, Magdeburg, Germany, ou_persistent22              
6German Center for Neurodegenerative Diseases, Magdeburg, Germany, ou_persistent22              
7High Magnetic Field Laboratory, Chinese Academy of Sciences, Hefei, China, ou_persistent22              

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Free keywords: NMR; Rat brain; Subcellular fractions; Protein; MRI phase contrast; Iron; Susceptibility; Exchange
 Abstract: Purpose Investigation of magnetic susceptibility and chemical exchange as sources of MRI phase contrast between gray and white matter resulting from protein and iron content from subcellular fractions. Methods This study analyzes the iron and macromolecule content of different subcellular fractions from rat brain and their relation to the water-resonance frequency by NMR spectroscopy. Additionally, the contributions of susceptibility and exchange were determined with different NMR reference substances. Results Only weak correlations between iron (r = 0.4318, P = 0.76) or protein content (r = 0.4704, P = 0.70) and frequency shift were observed. After membrane depletion, the correlation for iron increased to r = −0.9006 (P = 0.0009), whereas the shift relative to protein content increased much less (r = −0.4982, P = 0.64). Exchange-driven frequency shifts were 1.283 ppb/(mg/ml) for myelin and 0.775 ppb/(mg/ml) for synaptosomes; susceptibility-driven shifts were −1.209 ppb/(mg/ml) for myelin and −0.368 ppb/(mg/ml) for synaptosomes. The ratios between susceptibility and exchange differ significantly from simple protein solutions. Conclusions As a result of counteracting susceptibility and exchange and increased relative shifts in membrane-depleted fractions, we conclude that tissue microstructure accounts more for the in vivo phase contrast than in the situation of homogenized tissue. Thus, membranes may generate much of the in vivo MR phase contrast resulting from anisotropy. Magn Reson Med, 2016. © 2016 Wiley Periodicals, Inc.

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Language(s): eng - English
 Dates: 2016-05-032015-12-182016-05-042016-06-072017-05
 Publication Status: Published in print
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 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1002/mrm.26288
BibTex Citekey: MRM:MRM26288
PMID: 27271468
Other: Epub 2016
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Funding organization : Federal State of Saxony‐Anhalt
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Grant ID : 1211080005
Funding program : European Regional Development Fund
Funding organization : European Commission (EC)

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Title: Magnetic Resonance in Medicine
Source Genre: Journal
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Publ. Info: New York : Wiley-Liss
Pages: - Volume / Issue: 77 (5) Sequence Number: - Start / End Page: 2028 - 2039 Identifier: ISSN: 0740-3194
CoNE: https://pure.mpg.de/cone/journals/resource/954925538149