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  Hair cells use active zones with different voltage dependence of Ca2+ influx to decompose sounds into complementary neural codes.

Ohn, T. L., Rutherford, M. A., Jing, Z., Jung, S., Duque-Afonso, C. J., Hoch, G., et al. (2016). Hair cells use active zones with different voltage dependence of Ca2+ influx to decompose sounds into complementary neural codes. Proceedings of the National Academy of Sciences of the United States of America, 113(32), E4716-E4725. doi:10.1073/pnas.1605737113.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002B-14AB-D Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002C-6770-0
Genre: Journal Article

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 Creators:
Ohn, T. L., Author
Rutherford, M. A., Author
Jing, Z., Author
Jung, S., Author
Duque-Afonso, C. J., Author
Hoch, G., Author
Picher, M. M., Author
Scharinger, A., Author
Strenzke, A., Author
Moser, T.1, Author              
Affiliations:
1Research Group of Synaptic Nanophysiology, MPI for Biophysical Chemistry, Max Planck Society, ou_2205655              

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Free keywords: auditory system; dynamic range; presynaptic heterogeneity; spiral ganglion neuron; synaptic strength
 Abstract: For sounds of a given frequency, spiral ganglion neurons (SGNs) with different thresholds and dynamic ranges collectively encode the wide range of audible sound pressures. Heterogeneity of synapses between inner hair cells (IHCs) and SGNs is an attractive candidate mechanism for generating complementary neural codes covering the entire dynamic range. Here, we quantified active zone (AZ) properties as a function of AZ position within mouse IHCs by combining patch clamp and imaging of presynaptic Ca2+ influx and by immunohistochemistry. We report substantial AZ heterogeneity whereby the voltage of half-maximal activation of Ca2+ influx ranged over ∼20 mV. Ca2+ influx at AZs facing away from the ganglion activated at weaker depolarizations. Estimates of AZ size and Ca2+ channel number were correlated and larger when AZs faced the ganglion. Disruption of the deafness gene GIPC3 in mice shifted the activation of presynaptic Ca2+ influx to more hyperpolarized potentials and increased the spontaneous SGN discharge. Moreover, Gipc3 disruption enhanced Ca2+ influx and exocytosis in IHCs, reversed the spatial gradient of maximal Ca2+ influx in IHCs, and increased the maximal firing rate of SGNs at sound onset. We propose that IHCs diversify Ca2+ channel properties among AZs and thereby contribute to decomposing auditory information into complementary representations in SGNs.

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Language(s): eng - English
 Dates: 2016-07-26
 Publication Status: Published online
 Pages: -
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 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1073/pnas.1605737113
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Title: Proceedings of the National Academy of Sciences of the United States of America
Source Genre: Journal
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Pages: - Volume / Issue: 113 (32) Sequence Number: - Start / End Page: E4716 - E4725 Identifier: -