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  Interchain and intrachain crosslinking of actin thiols by a bifunctional thiol reagent

Faulstich, H., Heintz, D., & Drewes, G. (1992). Interchain and intrachain crosslinking of actin thiols by a bifunctional thiol reagent. FEBS Letters, 302(3), 201-205. doi:10.1016/0014-5793(92)80440-R.

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FEBSLett_302_1992_201.pdf (Any fulltext), 514KB
 
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 Creators:
Faulstich, Heinz1, Author              
Heintz, Daniela2, Author              
Drewes, Gerard2, Author              
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1Department of Molecular Cell Research, Max Planck Institute for Medical Research, Max Planck Society, ou_1497703              
2Max Planck Institute for Medical Research, Max Planck Society, ou_1125545              

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Free keywords: Unpolymerizable actin; Disulfide exchange reaction; Protein thiol; Reversible thiol-specific crosslink
 Abstract: From 1,9-nonylenedithiol and Ellman's Reagent the bifunctional asymmetric disulfide n-nonylene-1,9-bis-[5-dithio-(2-nitrobenzoic acid)] (NBDN) was prepared. By monovalent reaction with cysteine-374 the crosslinker could be introduced into monomeric actin, with release of one equivalent of yellow 2-nitro-5-thiobenzoate (NTB). From the monovalent actin derivative we prepared a crosslinked actin dimer (Cys-374-Cys-374') as well as a monomer with a crosslink between Cys-374 and Cys-10. Neither crosslinked actin species was able to polymerize the crosslinked monomer even in the presence of phalloidin. The crosslinked monomer polymerized on the addition of dithiothreitol, thus providing the first unpolymerizable actin species whose polymerizability can be restored under mild conditions. We suggest the use of NBDN as a thiol-specific crosslinker that reacts under spectrophotometric control and can be removed by the addition of thiols.

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Language(s): eng - English
 Dates: 1992-03-182002-01-161992-03-18
 Publication Status: Published in print
 Pages: 5
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 Table of Contents: -
 Rev. Type: Peer
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Title: FEBS Letters
  Other : FEBS Lett.
Source Genre: Journal
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Publ. Info: Amsterdam : Elsevier
Pages: - Volume / Issue: 302 (3) Sequence Number: - Start / End Page: 201 - 205 Identifier: ISSN: 0014-5793
CoNE: https://pure.mpg.de/cone/journals/resource/954925399501