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  PHF13 is a molecular reader and transcriptional co-regulator of H3K4me2/3

Chung, H.-R., Xu, C., Fuchs, A., Mund, A., Lange, M., Staege, H., et al. (2016). PHF13 is a molecular reader and transcriptional co-regulator of H3K4me2/3. eLife, 5: 5:e10607. doi:10.7554/eLife.10607.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002B-16E5-8 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002B-16E6-6
Genre: Journal Article

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 Creators:
Chung, Ho-Ryun1, Author              
Xu, Chao, Author
Fuchs, Alisa, Author
Mund, Andreas, Author
Lange, Martin, Author
Staege, Hannah , Author
Schubert, Tobias, Author
Bian, Chuanbing , Author
Dunkel, Ilona1, Author              
Eberharter, Anton, Author
Regnard, Catherine , Author
Klinker, Henrike, Author
Meierhofer, David2, Author              
Cozzuto, Luca, Author
Winterpracht, Andreas, Author
Di Croce, Luciano , Author
Min, Jinrong , Author
Will, Hans, Author
Kinkley, Sarah1, Author              
Affiliations:
1Computational Epigenetics (Ho-Ryun Chung), Independent Junior Research Groups (OWL), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1479658              
2Mass Spectrometry (Head: David Meierhofer), Scientific Service (Head: Christoph Krukenkamp), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1479669              

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Free keywords: PHD domain; PRC2; RNA polymerase II; biophysics; chromosomes; genes; human; molecular reader and effector; mouse; structural biology; transcriptional regulator
 Abstract: PHF13 is a chromatin affiliated protein with a functional role in differentiation, cell division, DNA damage response and higher chromatin order. To gain insight into PHF13's ability to modulate these processes, we elucidate the mechanisms targeting PHF13 to chromatin, its genome wide localization and its molecular chromatin context. Size exclusion chromatography, mass spectrometry, X-ray crystallography and ChIP sequencing demonstrate that PHF13 binds chromatin in a multivalent fashion via direct interactions with H3K4me2/3 and DNA, and indirectly via interactions with PRC2 and RNA PolII. Furthermore, PHF13 depletion disrupted the interactions between PRC2, RNA PolII S5P, H3K4me3 and H3K27me3 and resulted in the up and down regulation of genes functionally enriched in transcriptional regulation, DNA binding, cell cycle, differentiation and chromatin organization. Together our findings argue that PHF13 is an H3K4me2/3 molecular reader and transcriptional co-regulator, affording it the ability to impact different chromatin processes.

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Language(s): eng - English
 Dates: 2016-05-25
 Publication Status: Published online
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: -
 Identifiers: DOI: 10.7554/eLife.10607
 Degree: -

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Title: eLife
Source Genre: Journal
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Publ. Info: Cambridge : eLife Sciences Publications
Pages: - Volume / Issue: 5 Sequence Number: 5:e10607 Start / End Page: - Identifier: Other: 2050-084X
CoNE: /journals/resource/2050-084X